Banca de DEFESA: UIARA MARIA DE BARROS LIRA LINS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : UIARA MARIA DE BARROS LIRA LINS
DATE: 10/06/2025
TIME: 14:00
LOCAL: Universidade Católica de Pernambuco - UNICAP
TITLE:

BIOSYNTHESIS OF THE HMG-COA REDUCTASE INHIBITOR AND COPRODUCTION OF BIOEMULSIFIER BY Aspergillus spp. USING SUBSTRATES ALTERNATIVES.

KEY WORDS:

Statins; Filamentous fungi; Alternative substrates; Bioemulsifier.

PAGES: 181
BIG AREA: Outra
AREA: Multidisciplinar
SUBÁREA: Biotecnologia
SUMMARY:

HMG-CoA reductase (3-hydroxy-3-methylglutaryl-coenzyme A reductase) is the ratelimiting enzyme in cholesterol biosynthesis. Among its inhibitory molecules, statins stand out as a class of drugs widely used in the treatment of atherosclerosis and in reducing elevated cholesterol levels, becoming essential for the primary and
secondary prevention of coronary artery disease. In addition, they exhibit significant pleiotropic effects, which makes HMG-CoA reductase inhibitors strategic products that should be produced nationally to strengthen the development of the national health industrial complex. In this context, the objective of this study was to evaluate the biotechnological potential of Aspergillus niger UCP 1064 in the coproduction of HMG-CoA reductase inhibitors
and bioemulsifier through submerged fermentation using starch and cassava wastewater (manipueira) as alternative substrates. For biomolecule identification, a bioassay was performed as a screening method, followed by UV-visible spectrophotometry and thin-layer chromatography (TLC), and subsequent purification steps. The results indicated a production of 243.60 μg/mL and a yield of 1.91 g/L of statins, demonstrating that starch and manipueira are ideal substrates for the coproduction of statins and surfactants. The surfactant showed a surface tension (ST) of 49.4 mN/m and an emulsion index (EI24) of 95.25%. The statin exhibited antimicrobial activity against Gram-negative and Gram-positive bacteria as well as yeasts, highlighting its high potential in addressing the challenge of antimicrobial multiresistance. Cytotoxic analysis revealed a low hemolytic effect (6.47% and α-hemolysis). These results demonstrate that A. niger UCP 1064 is a promising strain, and that the tested substrates are viable, low-cost alternatives for the coproduction of statins and bioemulsifier with strong potential for pharmaceutical industry applications.

 

COMMITTEE MEMBERS:
Presidente - ***.501.554-** - GALBA MARIA CAMPOS TAKAKI - UNICAP
Interna - ***.451.344-** - JULIANA MOURA DE LUNA - UFPE
Interna - RAQUEL PEDROSA BEZERRA
Externo ao Programa - 1646614 - MARCOS ANTONIO BARBOSA DE LIMA - UFRPEExterna à Instituição - JACIANA DOS SANTOS AGUIAR - UFPE