Banca de DEFESA: ANNELIESE GONÇALVES COSTA MARINHO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANNELIESE GONÇALVES COSTA MARINHO
DATE: 30/05/2025
TIME: 09:00
LOCAL: Google Meet
TITLE:

Composition of inhalable pharmaceutical formulation and its production process, based on an oil/water nanoemulsion, containing corn oil rich in cannabidiol (CBD) for the treatment of LPS-induced pneumonia in Albino swiss mice

KEY WORDS:

Respiratory System, LPS, Inflammation, Nanoemulsion, CBD.

PAGES: 129
BIG AREA: Outra
AREA: Multidisciplinar
SUBÁREA: Biotecnologia
SUMMARY:

In severe acute respiratory syndrome (SARS), the inflammatory reaction in the pulmonary parenchyma can lead to death. Cannabidiol has demonstrated good anti-inflammatory activity due to its ability to bind to cannabinoid receptors in inflammatory cells. Based on this premise, the present study aimed to induce an acute inflammatory reaction with the nasal instillation of LPS in male Albino Swiss mice and subsequently treat these animals with CBD formulated in three different concentrations (0.05%, 0.1%, and 0.2%), using a nanoemulsion based on CBD-rich corn oil and converted into a nanotechnological pharmaceutical form for nebulization. In the production process, separate homogenization of the oil and aqueous phases was carried out. These were combined and initially mixed at 1500 rpm for 30 minutes, followed byt 500 rpm for 20 hours. After this processing, the Zeta test was performed to determine vesicle size and stability. According to the results, the average vesicle diameter was 107.6 nm, with a polydispersion index of 0.93 mV. Experimental groups were defined according to the CBD concentrations in the nebulization solution and the pulmonary parenchyma evaluation times (8, 12, and 24 hours). The negative control group animals were nebulized with 0.9% NaCl at the specified time intervals. The positive control group received methylprednisolone (MPD) at a dose of 30 mg/kg intraperitoneally for three days. After euthanasia, the lungs were routinely processed for paraffin embedding and stained with H-E. The pulmonary parenchyma was evaluated qualitatively and quantitatively to assess the inflammatory reaction and alveolar area, respectively. In the histopathological results of the pulmonary parenchyma, a decrease in mononuclear lymphoplasmacytic neutrophilic inflammatory infiltration was observed in the interalveolar septa, peribronchial bronchial interstitium at all three CBD concentrations (0.05%, 0.1%, and 0.2%) within 8 hours over three days of treatment. At the 12-hour interval, CBD concentrations of 0.1% and 0.2% were able to reduce the interstitial inflammatory infiltration compared to other treatments. At the 24-hour interval over three days, nebulization with CBD at all three concentrations was not effective in reducing interstitial inflammatory infiltration. The 30 mg/kg MPD intraperitoneal dose effectively reduced the pulmonary inflammatory process. The alveolar area (μm²) in the LPS+ group nebulized with 0.9% saline solution was 75% larger compared to animals nebulized with CBD at 8 or 12-hour intervals for three days. The treatment at a 24-hour interval increased the alveolar area by 52% in the LPS+/0.9% saline group compared to the treatments with CBD at concentrations of 0.05%, 1.0%, and 2.0%. The nanotechnological nebulization solution for mice induced with pulmonary inflammation by LPS was more effective in reducing inflammation and pulmonary emphysema in animals treated every 8 hours for three days.

COMMITTEE MEMBERS:
Interna - ANA LUCIA FIGUEIREDO PORTO
Externo à Instituição - BRUNO MENDES TENORIO - UFPE
Externa à Instituição - IVONE ANTONIA DE SOUZA - UFPE
Externa à Instituição - TEREZINHA CARLA CARVALHO DA SILVA
Presidente - VALDEMIRO AMARO DA SILVA JUNIOR