Banca de DEFESA: CAMILA FREIRE DE MELO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CAMILA FREIRE DE MELO
DATE: 28/05/2025
TIME: 15:00
LOCAL: Google Meet
TITLE:

BIOTECHNOLOGICAL POTENTIAL OF Salicornia neei IN MOLECULES WITH FUNCTIONAL PROPERTIES AND IN THE INDUCTION OF ROOTING BY CHITOSAN (isolated from Litopenaeus vannamei)

KEY WORDS:

Biopolymer. Green salt. Antioxidant activity. Biocompatibility. Rooting. plant growth

PAGES: 95
BIG AREA: Outra
AREA: Multidisciplinar
SUBÁREA: Biotecnologia
SUMMARY:

Sea asparagus, Salicornia neei, a halophyte plant native to Brazil, is widely known for its use in human nutrition as green salt, in addition to presenting important secondary metabolites with antioxidant, antimicrobial and antitumor properties, which constitute it as a functional plant. However, agricultural producers find it difficult to finance Salicornia, due to losses during cultivation and the complexity of finding them on the market. Therefore, to increase the use of S. neei as food, biotechnological strategies involving plant productivity are suggested. In this sense, investigations are carried out with the aim of expanding knowledge on active biomolecules of nutritional importance in S. neei, as well as evaluating the influence of the biopolymer chitosan as a rooting inducer. Initial studies were carried out evaluating the content of minerals and pigments (chlorophyll and carotenoids) in the fresh aerial part of the plant. Then, S. neei extracts were prepared with organic solvents (milli-q water, ethanol and ethanol - acetone 1:1), using different parts of the plant (leaf, stem and root), performing a physicochemical characterization and action antioxidant and antimicrobial. For antioxidant activity, the DPPH free radical method was used, antimicrobial activity by the disk diffusion method using bacteria (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella sp., Pseudomonas aeruginosa) and fungi (Candida albicans, C. guilliermondii, C .tropical). The chitosan polysaccharide with degrees of deacetylation above 90% was obtained from chitin extracted from shrimp exoskeleton residues (body and head), applying a new and efficient methodology described in the Patent application (BR102024002181-9). in use as a rooting inducer in Salicornia neei described in the patent application (BR1020240026187). The results obtained with S. neei, demonstrated a higher content of sodium (6.70%) and potassium (13.96%), in smaller quantities N, Ca, Mg, P, Fe, Mn. The extracted pigments were inspired by Chlorophyll B (4.76 mg/g), Chlorophyll A (2.95 mg/g) and Carotenoids (2.42 mg/g). The solvent system with the highest solubility of active molecules with antioxidant activity was ethanol - acetone (1:1), providing the highest antioxidant activity 97% for the stem, at a concentration of 24 mg/mL. Therefore, studies carried out with S. neEi extracts demonstrated promising results with nutritional composition, biomolecules with antioxidant and antimicrobial action, corroborating the reports described in the literature. Furthermore, the degree of deacetylation extracted from shrimp was the highest ever described in the literature and thus constitutes a new effective methodology and quality bioproduct. The application of this bioproduct produced favorable results as a rooting inducer in Salicornia neei, enabling propagation and future applications in other cultivars, thus exploring the use of the biopolymer chitosan as a biostimulant for plant growth and rooting

COMMITTEE MEMBERS:
Presidente - ***.501.554-** - GALBA MARIA CAMPOS TAKAKI - UNICAP
Interna - RAQUEL PEDROSA BEZERRA
Externo ao Programa - 1646614 - MARCOS ANTONIO BARBOSA DE LIMA - UFRPEExterno à Instituição - ALDO TORRES SALES - IPA
Externa à Instituição - DAYANA MONTERO RODRÍGUEZ - UNICAP