SUSTAINABLE PRODUCTION OF BIOPIGMENTS BY Talaromyces spp. USING RENEWABLE SUBSTRATES AND
APPLICATION IN THE TEXTILE INDUSTRY.
Dyeing, Biopigments, Filamentous Fungus, Renewable substrates, Caatinga.
The global demand for natural pigments and dyes has increased considerably in recent years, driven by a heightened awareness of the toxic effects of various synthetic dyes on human health and the environment, in the dyeing of textile products, due to the problems associated with discharge of artificial dyes with mutagenic, carcinogenic and teratogenic action. Therefore, the search for natural dyes becomes a viable alternative, aiming at reducing environmental impacts, mainly to water resources. Thus, natural pigments produced by fungi are attractive and promising, due to their intrinsic properties (natural compound, color strength) and the wide range of colors associated with the bioactivity property. However, the microbiological production of these pigments has a high cost, due to conventional substrates, which can increase the cost of the “Upstream” stage by about 50% in industrial processes. In turn, the generation of agro-industrial residues has been growing and contributing to increase the pollution of the environment, the loss of materials with potential for application and increasing the degradation of ecosystems. In this context, studies are being carried out with the objective of investigating the production of pigments by samples of the genus Talaromyces spp., isolated from soils of the caatinga biome, using as conventional sources for a better understanding of the metabolism of microorganisms, for the next steps of the use alternative sources. As alternative sources, sugarcane bagasse, residual glycerol from the production of biodiesel and lignocellulolytic material from forage palm, from the production of ethanol will be used as sources of carbon. As a source of nitrogen, maize, obtained from the industrial processing of maize, will be used in the formulation of growing media, aiming at reducing production costs through the concepts of biocircular economy. The investigations are being carried out through the bioprospecting of Talaromyces samples, evaluating the radial growth and pigment production in different carbon sources (sucrose, fructose, glucose, maltose and starch), as well as different salinity concentrations (0.5%, 1%, 2%, 4% and 6%), kept at room temperature of 28ºC, as inducers of biopigments production in Talaromyces. The results obtained indicated that bioprocesses were developed for the production of pigments by submerged fermentation using agro-industrial residues with a composition close to the selected carbon source, under different concentrations and evaluating the parameters pH, temperature, luminosity and aeration, evaluating the fungal growth and production of pigments. In the next step, the cultivation conditions in a bioreactor for pigment production will be established, analyzing the influence of aeration and agitation, using complete factorial designs, with variable response to biomass production and pigment production. Stability and toxicity of the produced pigments will also be analyzed. The final stage of the research will consist in the extraction, characterization and identification of the produced pigments, and evaluation of the potential in the dyeing of fabrics of synthetic and natural fibers. After dyeing, the fabric samples will be evaluated through the washability test, fasteners, mordants, among others. Dyeing with natural pigments will represent a scientific and technological advance for the production of textile articles, representing an innovative and promising alternative for the textile area in the use of biopigments without toxicity to the environment and man.