Banca de QUALIFICAÇÃO: EDMO HENRIQUE MARTINS CAVALCANTE

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : EDMO HENRIQUE MARTINS CAVALCANTE
DATE: 05/08/2022
TIME: 09:00
LOCAL: google meet
TITLE:

CHITOSAN AND MAGNETITE BASED COMPOUNDS FOR DNA ADSORPTION  AND APPLICATIONS IN ZnO-MODIFIED ANTIBACTERIALS.

KEY WORDS:

adsorption, electrospinning, decontamination, wound dressing.

PAGES: 147
BIG AREA: Outra
AREA: Multidisciplinar
SUBÁREA: Biotecnologia
SUMMARY:

The isolation of nucleic acids, a step that precedes several biochemical and diagnostic processes, is time consuming, whereas the growing demand for genomic analysis requires the development of alternative systems for biomolecule extraction. The magnetic separation of DNA has aroused more interest because it allows its extraction from complex media by using a suitable buffer system. However, these media may include antibiotic resistance genes that are overlooked as a pollutant in contaminated effluents and fluids, which favors the emergence of resistant strains. These pathogenic organisms, although structurally simple, have a highly plastic genome, which contributes to their diversification and adaptation through the dissemination of resistance, virulence, and metabolic pathways genes. As an alternative to conventional antimicrobials, nanotechnology explores the development of particles that interact with the cell envelope of the prokaryote, leading to the leakage of cytosolic material and the diffusion of reactive species. In this work, magnetized Eudragit E-100 fibers and magnetite nanoparticles functionalized with chitosan were produced for DNA adsorption. In due course, antibacterial composites were produced using the biopolymer and zinc oxide. The adsorption process by the fibers was explained by the Freundlich isotherm, with a maximum capacity of 55.14 mg.g-1 obtained experimentally. The pseudo-second-order kinetic model accurately described the mass transfer and the porous nature of the material led to consider the Weber-Morris intraparticle diffusion model as well. Chitosan- functionalized magnetite showed a higher adsorption capacity, estimated at 80.26 mg.g-1 according to the Langmuir model. Chitosan-based composites in membrane shape and incorporated into the skin of Nile tilapia (Oreochromis niloticus) presented mechanisms that reduced the death time and offered a satisfactory degree of inhibition of the Staphylococcus aureus biofilm (ATCC 25923).

BANKING MEMBERS:
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