Banca de QUALIFICAÇÃO: THALLES CLOVES MACIEL DE MOURA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : THALLES CLOVES MACIEL DE MOURA
DATE: 30/07/2022
TIME: 14:00
LOCAL: google meet
TITLE:

EFFECT OF ADDITION OF CHITOSAN IN FREEZING DILUENT OF STALLIONS SEMEN.

KEY WORDS:

Carboxymethylchitosan, chitosan, cryopreservation, chitin, semen.

PAGES: 59
BIG AREA: Outra
AREA: Multidisciplinar
SUBÁREA: Biotecnologia
SUMMARY:

The aim of this study was to investigate the effects of the addition of carboxymethylquinones (CQ) on the quality of post-thawed semen. 5 ejaculates were obtained from 4 stallions, twice a week, totaling 20 ejaculates. Then, it was diluted (20 x 106 spz/mL) in a freezing medium, the commercial one and supplemented with CQ divided into 4 experimental groups: control, 0.75mg/mL, 1.5mg/mL and 3mg/mL. The groups were packaged in straws (0.5 mL) and secured to freezing and storage at -196°C. The thawing of the straws was performed at 37°C/3 and the groups were studied for kinetics, plasma membrane and transversely mal and mitochondrial potential. Statistical analysis was performed and results were expressed as mean ± standard error of the mean and as significant differences when P < 0.05. The addition of 0.75 mg/mL of CQ to the extender showed no significant difference (P < 0.05) in MT and VCL compared to the control group, but the groups treated with 1.5 and 3 mg/mL were lower. (P < 0.05). As for the parameters RAP, VSL and VAP, there was no difference between the control, 0.75 and 1.5 mg/mL, but it was superior to the group with 3mgmL. As for the fluorescence microscopy analysis, there was a difference of 3mg/mL in the iAC in the treated group, which had lower percentages of intact acrosomes. In WOB, the control group did not differ from 0.75mg/mL but was superior to the 1.5 and 3mg/mL groups. The parameters of STR, ALH, DCF. Membrane integrity and mitochondrial potential had no significant differences. More studies need to be done to investigate the action of other chitosan results on the quality of thawed equine semen.

BANKING MEMBERS:
Externo à Instituição - DIOGO RIBEIRO CAMARA - UFAL
Externa à Instituição - SILDIVANE VALCACIA SILVA - UFPB
Interna - 1803550 - TATIANA SOUZA PORTO