Banca de DEFESA: CHARLES BRONZO BARBOSA FARIAS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CHARLES BRONZO BARBOSA FARIAS
DATE: 14/06/2022
TIME: 14:00
LOCAL: IATI - Instituto Avançado de Tecnologia e Inovação
TITLE:

PRODUCTION OF GREEN SURFACTANTS ON A SEMI-INDUSTRIAL SCALE FOR APPLICATION  IN THE REMOVAL

OF HYDROPHOBIC POLLUTANTS (BIOEESEMI).

KEY WORDS:

green surfactants; biosurfactant, Pseudomonas, detergent, petroleum, heavy oil, organic pollutants.

PAGES: 220
BIG AREA: Outra
AREA: Multidisciplinar
SUBÁREA: Biotecnologia
SUMMARY:

Science has contributed a lot to the advancement of technology and innovation in production processes and their applications. In this context, obtaining cleaning products becomes indispensable in different professional environments, especially in the industrial sector. Industrial plants powered by heavy oil have problems with leaks in different parts of the system, such as during oil transport, lubrication of engines and equipment, and mechanical failures. Most surfactants, surfactants, degreasing agents, and solvents that make up the formulation of detergents commonly used for cleaning grease-covered surfaces are synthetic, non-biodegradable and toxic, posing risks to the environment and the health of workers involved in the process. cleaning process. In this sense, green surfactants, of a biodegradable nature and reduced toxicity, synthesized from renewable raw materials by chemical modification (renewable-based surfactants) or derived from microorganisms (biosurfactants) and plants (vegetable surfactants), have been widely studied to replace chemical surfactants as attractive and efficient solutions in decontamination processes. In this work, initially, strains of the bacteria Pseudomonas cepacia CCT 6659, Pseudomonas aeruginosa UCP 0992, Pseudomonas aeruginosa ATCC 9027 and Pseudomonas aeruginosa ATCC 10145 were evaluated as producing biosurfactants in media containing different combinations and types of substrates under different culture conditions. The biosurfactant produced by P. aeruginosa ATCC 10145 grown in a mineral medium composed of 5.0% glycerol and 2.0% glucose was selected to formulate a biodetergent capable of removing heavy oil. The biosurfactant was able to reduce the surface tension of the medium to 26.40 mN/m, with a yield of approximately 12.00 g/L. The biosurfactant emulsified 97.40% and dispersed 98.00% of the engine oil. Then, the biodetergent formulated with 0.5% of the biosurfactant, 20% of natural solvent, 2.0% of fatty alcohol and 0.5% of stabilizing gum was evaluated against the microcrustacean Artemia salina and the seeds of the vegetable Brassica oleracea, demonstrating low toxicity. The detergent was also compared with commercial formulations. The stability of the biodetergent was evaluated for volumetric variations (4 - 10 liters) and stirring times (5 - 10 minutes) at 3200 rpm at 80°C. The long-term stability (365 days) and the removal efficiency of OCB1 fuel oil from floors and metal surfaces were also evaluated. The results showed that the interaction between the processing conditions influenced the final characteristics of the product, with emphasis on the stirring time of 7 minutes, for which approximately 100% stability of the formulation was reached. The biodetergent showed high efficiency, with results of 100% removal of OCB1 oil impregnated on the surfaces. In the last stage, the scale-up of biodetergent production was carried out in an industrial pilot unit of 600 L per batch built based on the improvement of the formulation on a laboratory scale. The biodetergent obtained on a large scale showed biodegradability, absence of toxicity, stability and maximum efficiency in the removal of OCB1 oil (100%) under different application conditions and for different surfaces (metallic, plastic, concrete). In this way, the biodetergent produced was presented as a technological innovation for being a new product, not existing in the market. A preliminary final product cost study demonstrated that the biodetergent is viable for commercial application.

BANKING MEMBERS:
Externo à Instituição - SERGIO PERES RAMOS DA SILVA - UPE
Externa à Instituição - RAQUEL DINIZ RUFINO - UFPE
Externo à Instituição - MOHAND BENACHOUR - UFPE
Presidente - 735.801.634-34 - LEONIE ASFORA SARUBBO - UNICAP
Interno - 079.743.104-72 - VALDEMIR ALEXANDRE DOS SANTOS - UNICAP