Banca de QUALIFICAÇÃO: STEFANNY ARIELLE GOMES DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : STEFANNY ARIELLE GOMES DA SILVA
DATE: 27/04/2026
TIME: 09:00
LOCAL: Híbrida – Laboratório de Ecofisiologia e Comportamento Animal e Plataforma remota do google meet.
TITLE:

Gestational exposure to chlorpyrifos: Systematic mapping in biological matrices and assessment of embryotoxicity in the zebrafish (Danio rerio) model

KEY WORDS:

Keywords: Chlorpyrifos, Pregnancy, Maternal-fetal exposure, Biomonitoring, zebrafish.

PAGES: 55
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Chlorpyrifos is an organophosphate insecticide widely used in global agriculture and known for its potential toxic effects on human and environmental health. Despite regulatory restrictions in several countries, this compound remains present in crops across various regions, including Brazil. Concern is even greater during pregnancy, a period marked by intense biological vulnerability, in which maternal physiological changes can alter the absorption and metabolism of xenobiotics. Furthermore, scientific evidence indicates that chlorpyrifos and its metabolites are capable of crossing the placental barrier, leading to direct fetal exposure and increasing the risk of neurobehavioral and perinatal developmental impairments. In this context, the present study aimed to integrate evidence on gestational exposure to chlorpyrifos through systematic literature mapping and experimental assessment of maternal-fetal toxicity using the Zebrafish (Danio rerio) model. Initially, Systematic Literature Mapping was conducted in the PubMed, Scopus, and ScienceDirect databases, covering studies published between 2005 and 2026. After methodological screening and the application of inclusion and exclusion criteria, 33 original articles were selected. The results showed an increase in publications over the past decade, demonstrating growing international concern about maternal-fetal exposure to pesticides. Most studies were conducted in China and the United States, followed by European and Latin American countries. The main form of exposure described was non-occupational environmental, related to the consumption of contaminated food, water, household dust, air, and proximity to agricultural areas, showing that the risk is not limited to rural female workers. Urine was the most commonly used biological matrix for biomonitoring, with concentrations ranging from 0.021 to 6.7 µg/L. Maternal blood, umbilical cord blood, placenta, serum, and hair were also used. The most employed analytical techniques were LC-MS/MS and GC-MS/MS, highlighting high sensitivity in detecting chlorpyrifos and its main metabolite TCPy. Next, the toxic potential of the commercial formulation Klorplan 480 EC was experimentally assessed using zebrafish (Danio rerio) as an alternative model. Viable embryos were exposed by immersion to concentrations of 10, 100, 500, and 1000 µg/L in two distinct developmental periods: early exposure, between 2 and 24 hours post-fertilization (hpf), and late exposure, between 24 and 48 hpf. Organisms were monitored up to 144 hpf for mortality and teratogenic alterations. The results showed a dose-dependent response, with an increase in affected organisms at concentrations of 500 and 1000 µg/L. Among the main effects observed were yolk sac edema, pericardial edema, spinal deformities, delayed development, and embryonic mortality. Early exposure showed greater sensitivity compared to late exposure, indicating that the initial stages of embryogenesis represent a critical window for the toxic action of the pesticide. In this way, the results demonstrated that chlorpyrifos poses a significant toxicological risk to pregnant women and fetuses, being widely detectable in human populations and capable of inducing relevant alterations in embryonic development.

COMMITTEE MEMBERS:
Presidente - PABYTON GONCALVES CADENA
Externo ao Programa - 2140782 - ALEX SOUZA MORAES - UFRPEExterna à Instituição - SÂMARA DA SILVA GOMES - UFPE