Banca de DEFESA: DENNY PARENTE DE SÁ BARRETO MAIA LEITE

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DENNY PARENTE DE SÁ BARRETO MAIA LEITE
DATE: 15/12/2025
TIME: 08:00
LOCAL: Departamento de Medicina Veterinária – Universidade Federal Rural de Pernambuco
TITLE:

Ecology of antimicrobial resistance in wildlife: Clinical implications and connections between One Health and conservation medicine.

KEY WORDS:

Bacterial resistance to multiple drugs. Bacterial genetics. Bacterial biofilm. Epidemiological surveillance. Conservation medicine.

PAGES: 222
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Antimicrobial resistance (AMR) in bacteria associated with wildlife is an emerging challenge with a limited number of studies, especially in rehabilitation centers and anthropogenically modified aquatic ecosystems. Therefore, this study investigated the phenotypic and genotypic resistance profiles, multiple antibiotic resistance (MAR) indices, and biofilm formation in Staphylococcus spp. and Mammaliicoccus sciuri isolated from Neotropical Non-Human Primates, other wild mammals and reptiles undergoing rehabilitation in Northeast Brazil, and from Inia araguaiaensis river dolphins in the Amazon. Rectal samples were collected from 20 Sapajus libidinosus undergoing rehabilitation, oropharyngeal and rectal samples from 84 wild mammals of 15 species, oropharyngeal and cloacal samples from 87 reptiles of six species, and skin lesion samples from eight river dolphins, with bacterial isolation on Mannitol Salt agar. Identification was performed by matrix-assisted laser desorption/ionization mass spectrometry and time-of-flight spectroscopy, and susceptibility to different antimicrobials was evaluated by the disk-diffusion method. Detection of resistance genes to beta-lactams, macrolides, tetracyclines, and fluoroquinolones was performed by polymerase chain reaction, and quantification of biofilm formation by the crystal violet method was applied to subsets of isolates. In the set of studies, 188 isolates were obtained, with a predominance of Mammaliicoccus sciuri (120/188; 63.8%). In Sapajus libidinosus, 19 isolates were recovered, with resistance to penicillin in 12/19 (63.2%) and to tetracycline in 11/19 (57.9%), multidrug resistance in 4/19 (21.1%) and detection of the genes tetM in 7/19 (36.8%), tet(38) in 6/19 (31.6%), blaZ in 5/19 (26.3%), msrA in 5/19 (26.3%) and mecA in 1/19 (5.3%). In wild mammals, bacterial growth was observed in 63/84 (75.0%) animals, with 63 isolates, of which 32/63 (50.8%) were resistant to at least one antimicrobial and 12/63 (19.0%) were multidrug-resistant. Resistance to cefoxitin was observed in 18/63 (28.6%), erythromycin in 17/63 (27.0%), tetracycline in 16/63 (25.4%), and clindamycin in 14/63 (22.2%), with MAR indices ranging from 0.00 to 1.00 (mean 0.21) and biofilm formation in 33/63 (52.4%) isolates. In reptiles, 87 isolates were obtained, mostly M. sciuri (79/87; 90.8%), resistance to at least one antimicrobial was observed in 46/87 (52.9%) isolates, with resistance to oxacillin in 40/87 (46.0%) and erythromycin in 16/87 (18.4%), multidrug resistance in 12/87 (13.8%) and MAR index > 0.20 in 22/87 (25.3%). In Inia araguaiaensis, 19 isolates were recovered, with a predominance of S. aureus, S. warneri and S. epidermidis (4/19; 21.1% each), resistance to penicillin in 18/19 (94.7%) and multidrug resistance in 4/19 (21.1%). The genes blaZ, mecA, msrA, norC, tetL, and tetM were detected in 8/19 (44.4%), 2/19 (10.5%), 2/19 (10.5%), 2/19 (10.5%), 3/19 (15.8%), and 1/19 (5.3%) isolates, respectively, and biofilm formation was observed in 15/19 (78.9%) isolates, including 4/15 (26.7%) strong producers. Taken together, the results demonstrate that wild mammals and reptiles undergoing rehabilitation, as well as Amazon River dolphins, act as bioindicators of AMR in terrestrial and aquatic environments, reinforcing the need for microbiological surveillance and strengthening biosecurity measures in wildlife care centers.

COMMITTEE MEMBERS:
Presidente - RINALDO APARECIDO MOTA
Interna - TATIANA SOUZA PORTO
Externa ao Programa - 2625657 - MERCIA RODRIGUES BARROS - UFRPEExterna à Instituição - DEBORA ROCHELLY ALVES FERREIRA
Externo à Instituição - RAYLSON PEREIRA DE OLIVEIRA