Banca de QUALIFICAÇÃO: ELTON SANTOS GUEDES DE MORAIS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ELTON SANTOS GUEDES DE MORAIS
DATE: 15/09/2025
TIME: 14:00
LOCAL: Departamento de Biologia
TITLE:

Treatment of Calvarial Lesions in Rats with Micrografts Containing Mesenchymal Stem Cells Obtained by the Rigenera® System Associated with Biomaterials

KEY WORDS:

Stem cells; Bone defect; Cell therapy; Regenerative medicine; Biopolymer; Strong bone

PAGES: 50
BIG AREA: Ciências Biológicas
AREA: Biotecnologia
SUBÁREA: Biotecnologia em Saúde Humana e Animal
SPECIALTY: Ciências de Animais de Laboratórios
SUMMARY:

The application of cell therapy in regenerative medicine represents a significant innovation for the treatment of conditions in both animals and humans. This advancement aims to improve the quality of life of patients affected by various diseases, including critical-size bone defects. Several methodologies exist to induce bone repair and regeneration; however, regenerative medicine emerges as an innovative strategy, as it seeks to regenerate or replace damaged cells, organs, and tissues with the objective of restoring normal body function, shifting the conventional focus from transplants to therapeutic approaches anchored in the properties of stem cells. This study aims to evaluate the effectiveness of the association between micrografts containing stem cells and biomaterials such as cashew gum polymer and Strongbone® in the healing of bone lesions. For this purpose, 80 male Wistar rats, 90 days old, will be used. The animals will be randomly divided into five groups of 16 animals each: (i) CN – Control group; (ii) GC – Cashew gum polymer group; (iii) SB – Strongbone® group; (iv) RSB – Strongbone® associated with micrografts derived from the Rigenera® system; (v) RGC – Cashew gum polymer associated with micrografts derived from the Rigenera® system. To induce the lesion, a 1.5 cm craniocaudal incision will be performed, extending from a midpoint between the eyes to a midpoint between the ears. After incision of the skin and subcutaneous tissue in the calvarial region, a circular defect will be created in the median region between the parietal bones using a 5 mm trephine bur with a controlled-speed motor. In the control group, after creating the bone defect, the soft tissue and bone edges of the defect will be stimulated to promote controlled bleeding, followed by filling of the defect with a blood clot. In the remaining groups, the respective materials will be implanted in the defect area. At the end, histological and histochemical evaluations will be performed to analyze the bone repair process following treatment. This project, conceived in response to the urgent need for innovative therapies, proposes the use of cell therapy as a revolutionary tool in the treatment of rotator cuff injuries. In addition to offering an innovative approach, the project seeks to significantly reduce the healing and recovery time of patients affected by critical-size bone defects. Thus, it aims not only to provide new treatment perspectives but also to improve outcomes in terms of effectiveness and speed of recovery. The results of this study may contribute to advances in treatment by presenting an alternative therapeutic approach for bone lesions in both animals and humans.

COMMITTEE MEMBERS:
Presidente - ANISIO FRANCISCO SOARES
Externo ao Programa - 1929164 - FRANCISCO DE ASSIS LEITE SOUZA - UFRPEExterno ao Programa - 2279588 - MARCELO WEINSTEIN TEIXEIRA - nullExterna à Instituição - SHEILLA ANDRADE DE OLIVEIRA