Banca de QUALIFICAÇÃO: GUSTAVO DE OLIVEIRA ALVES PINTO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : GUSTAVO DE OLIVEIRA ALVES PINTO
DATE: 25/09/2025
TIME: 09:00
LOCAL: Auditório de Caprinos - DMV
TITLE:

Occurrence of Leptospira spp. in the reproductive tract of mares with reproductive failure in Pernambuco State, Brazil

KEY WORDS:

Equine Genital Leptospirosis; Leptospira; Epidemiology; ReproductiveDisorders; Horses 

PAGES: 51
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Leptospirosisis a globally distributed bacterial disease caused by bacteria of the genus Leptospira. In animal production systems, the circulation of Leptospira spp. with in herds is directly associated with economic losses, as infected animals often show reduced productivity. Genital leptospirosis is a reproductive syndrome linked to the colonization of reproductive organs by Leptospira spp., which may lead to reproductive failures such as embryonic death, abortion, and stillbirth. Despite its impact, no studies have focused on detecting Leptospira spp. in reproductive failures in the state of Pernambuco, Brazil. This study aimed to perform serological and molecular investigations of Leptospira spp. in mares withre productive failure. A total of 43 animals from 10 farms in Pernambuco and one aborted fetus (AF) were evaluated. For serological analysis, the Microscopic AgglutinationTest (MAT) was performed using a panel of 25 Leptospira spp. serovars. For molecular analyses, cervicovaginal mucus (CVM) samples from mares and a pool of organs from the AF were subjected to polymerase chain reaction (PCR) targeting the lipL32 gene, exclusive to pathogenic Leptospira, and positive samples were submitted to nested PCR targeting the SecY gene for sequencing. The CVM and the AF of one mare tested positive for the lipL32 gene, with sequencing confirming L. interrogans, representing the first detection of Leptospira in CVM and equine abortion in Pernambuco. Among the other animals, 92.8% (39/42) of mares tested seropositive to at least one Leptospira serogroup, with the Sejroe serogroup predominating (43.6%, 17/39). Regarding molecular analyses, 57.1% (24/42) of mares tested positive for thelipL32 gene in CVM. These findings highlightthe role of Leptospira spp. as an etiological agent of reproductive failure in Brazil and emphasize the need for preventive measures against this pathogen in the Northeast region.

COMMITTEE MEMBERS:
Presidente - RINALDO APARECIDO MOTA
Externa ao Programa - 1364514 - ANDREA ALICE DA FONSECA OLIVEIRA - UFRPEExterna ao Programa - 1076653 - RENATA PIMENTEL BANDEIRA DE MELO - UFRPE