Banca de QUALIFICAÇÃO: ELIZABETH SIMÕES DO AMARAL ALVES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ELIZABETH SIMÕES DO AMARAL ALVES
DATE: 17/03/2025
TIME: 10:00
LOCAL: Online via Google Meet
TITLE:

Development of an antimicrobial product based on multiple nanoemulsion with essential oil for subclinical animal mastitis.

KEY WORDS:

Animal Health, Nanotechnology, Dairy Farming, Phytotherapy, Dipping, Food Safety.

PAGES: 86
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Bovine mastitis is the leading disease and the primary reason for the use of antimicrobials in dairy farming, contributing to antimicrobial resistance and antibiotic residues in milk, posing a threat to public health. Thus, there is an urgent need for antimicrobial alternatives to address this issue. This study aimed to develop an antimicrobial product for use in pre- and post-dipping to control mastitis in dairy herds. To achieve this, a technological prospecting study was conducted in patent databases on innovations related to mastitis. The collected data revealed that most inventions proposed antimicrobial alternatives, but there was a noticeable gap in inventions based on natural products. Based on these findings, a production process for a multiple nanoemulsion-based antimicrobial incorporating essential oil was developed using high-energy emulsification. The best formulations were selected, with the physicochemical properties of EM-OM10 measuring 83.08 ± 0.76 nm in size, 0.21 PDI, and a zeta potential of -11.70 ± 0.61 mV, while EM-OS10 measured 138.70 ± 0.26 nm, 0.22 PDI, and a zeta potential of -11.70 ± 0.61 mV. Organoleptic tests, centrifugation, and thermal stress analyses demonstrated good stability. The antimicrobial efficacy was assessed by exposing the formulations for different time intervals (15", 30", 60", and 300") against Staphylococcus aureus, Staphylococcus coagulase-positive, and Escherichia coli from clinical isolates. The results indicated that the nanoemulsions exhibited significant antibacterial activity, with the highest efficacy observed at 300", reaching 65.71% for EM-OS, 68.57% for EM-OM, and 94.28% in the control group. At 60", efficacy decreased to 48.57% (EM-OS), 34.28% (EM-OM), and 74.28% (control group). At 30", activity was further reduced to 5.71% (EM-OS), 8.57% (EM-OM), and 54.28% (control group). At the shortest exposure time of 15", the nanoemulsions exhibited no antibacterial activity (0%), whereas the control group showed 22.85%. This study reinforces the potential of nanotechnological systems in developing new therapeutic strategies for dairy farming, offering effective alternatives to conventional antibiotics, contributing to food safety and public health.

COMMITTEE MEMBERS:
Presidente - PABYTON GONCALVES CADENA
Externo ao Programa - 1929164 - FRANCISCO DE ASSIS LEITE SOUZA - UFRPEExterna à Instituição - MARIA DO CARMO DE BARROS PIMENTEL - UFPE