Banca de DEFESA: MARIA CLARA FEITOSA DE ALBUQUERQUE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA CLARA FEITOSA DE ALBUQUERQUE
DATE: 27/02/2025
TIME: 10:00
LOCAL: https://meet.google.com/ren-gaea-uit)
TITLE:

Phenotypic and molecular characterization of Escherichia coli isolated from parrots (Amazona sp.) of illegal origin in Pernambuco

KEY WORDS:

Extended Spectrum Beta-lactamase; Diarrheagenic Escherichia coli; Zoonosis; Psittacidae; Wildlife Rehabilitation Center.

PAGES: 75
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Victims of human actions in the environment, wild animals rescued from illegal practices are commonly sent to the Wildlife Screening and Rehabilitation Centers (CETRAS) or zoos. The investigation of resistance and virulence traits is increasingly relevant in these institutions in all research involving microorganisms of epidemiological importance, especially when the impact falls on One Health. The objective of this dissertation was to characterize the production of biofilm, Extended Spectrum β-lactamases (ESBL) and virulence (Escherichia coli Diarrhea Occurrence - DEC) in Escherichia coli isolates from the intestinal microbiota of Amazona sp. from the Wildlife Screening and Rehabilitation Center (CETRAS Tangara), Recife, Pernambuco. Twenty-eight E. coli isolates were recovered and subjected to biofilm production assay, modified double disk synergism test (DDST) and molecular diagnosis by PCR for ESBL genes blaTEM-1, blaSHV-1 and CTX-M825 and virulence genes DEC of the Enteropathogenic (EPEC), Enterohemorrhagic (EHEC) and Shiga Toxin-Producing (STEC) classes, stx1, stx2, eaeA and HlyA. The clinical sign of diarrhea was observed exclusively in Resident birds (43.8%, 28/64). In the phenotypic tests, 100% (28/28) of the isolates produced biofilm and 32.1% (9/28) were positive in the DDST for ESBL production. In the genotypic tests, 13% (4/28) were positive for blaTEM-1 genes for ESBL production. The stx2 gene was detected in 13% (4/28) of the isolates, confirming the presence of STEC-type DEC in the birds investigated. The other genes were not detected. Five isolates were positive for two or more phenotypic and genotypic tests and three of these had genotypes for ESBL and STEC. It is concluded that Amazona aestiva and Amazona amazonica can be used as bioindicators of environmental quality for biofilm-producing E. coli, Extended Spectrum β-lactamases (E

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