Banca de QUALIFICAÇÃO: JÉSSICA DE CRASTO SOUZA CARVALHO REIS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JÉSSICA DE CRASTO SOUZA CARVALHO REIS
DATE: 10/02/2025
TIME: 10:00
LOCAL: https://meet.google.com/fgf-obbj-esk
TITLE:

GLANDERS IN EQUINES: GENOTYPIC CHARACTERIZATION, VIRULENCE ANALYSIS, ANTIMICROBIAL RESISTANCE OF Burkholderia mallei, AND EVALUATION OF A VACCINE PLATFORM WITH EXTRACELLULAR VESICLE TECHNOLOGY.

KEY WORDS:

Genotyping; Immunogen; Glanders; Zoonosis; Equids.

PAGES: 72
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Glanders is a zoonotic disease, with no available vaccine, that represents a significant economic and public health problem. Caused by the bacterium Burkholderia (B.) mallei, it mainly affects equids, presenting clinically with fever, cough, nasal discharge, and nodular lesions, which may progress to severe respiratory alterations. The bacterium is notable for its resistance to antibiotics, evasion of the immune system, and its historical use as a biological weapon. This study aimed to conduct the genotypic characterization, virulence, and resistance analysis of B. mallei isolates, as well as evaluate the efficacy of a vaccine for glanders in equids using extracellular vesicle technology. Initially, Burkholderia mallei isolation was performed, by identification through conventional PCR for the IS407-flip gene and Burk gene, whole-genome sequencing, identification of virulence and resistance genes and proteins, recovery and characterization of extracellular vesicles (EVs), transmission electron microscopy of EVs, in vitro analysis of the effect of EVs on THP-1 cell line-derived macrophages, in vitro analysis of the effect of EVs on lymphocytes, cell viability assay to evaluate EV cytotoxicity, and cytokine detection by flow cytometry. Two B. mallei isolates were obtained: BmBr01 and BmBr02, which presented similar virulence and resistance profiles, but BmBr02 stood out for its greater genetic diversity, suggesting higher adaptive plasticity. The EVs, with a predominant size between 100 and 200 nm, showed mitochondrial activity and induced a significant immune response, increasing the production of inflammatory cytokines such as IL6, TNF, and IFN-γ. These results indicate the potential of EVs as a vaccine platform against B. mallei, suggesting that the EVs derived from the bacterium induce a Th1-type response, as evidenced by the production of TNF and IFN-γ, differentiating from the response induced by inactivated bacteria, reinforcing the role of EVs as vaccine candidates.

COMMITTEE MEMBERS:
Presidente - RINALDO APARECIDO MOTA
Interno - JOSE WILTON PINHEIRO JUNIOR
Externa à Instituição - THAIS FERNANDA DE CAMPOS FRAGA DA SILVA