Banca de DEFESA: NATALY SAYONARA DA SILVA MELO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : NATALY SAYONARA DA SILVA MELO
DATE: 31/05/2024
TIME: 13:00
LOCAL: Sala de aula e meet (hibrida)
TITLE:

Phenotypic and molecular resistance profile of Staphylococcus aureus and Escherichia coli isolated from artisanal coalho cheese sold close to basic health units in Recife

KEY WORDS:

resistance genes, enterotoxins, mecA, MRSA, cefoxitin, basic health units

PAGES: 97
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Inspeção de Produtos de Origem Animal
SUMMARY:

Staphylococcus aureus and Escherichia coli are opportunistic microorganisms that play an important role in the spread of resistance to antimicrobials among humans, animals, the environment, and food, being considered an important public health problem. Culturally important food for the northeast region, which has been gaining ground in the rest of Brazil, is artisanal coalho cheese. However, as it is produced with raw milk and undergoes considerable manipulation in its production and commercialization, this product is more likely to be contaminated by these microorganisms. Thus, the objective of this work was to phenotypically and genotypically characterize strains of S. aureus and E. coli isolated from samples of artisanal coalho cheese sold in establishments located close to basic health units distributed in the eight health districts of the city of Recife - PE. A total of 129 samples were collected and suspected strains of S. aureus and E. coli were isolated. The identification of these isolates was carried out by biochemical tests and polymerase chain reaction, 16S rRNA technique, and Spatyping. Then, phenotypic antimicrobial resistance tests were carried out using disk diffusion and minimum inhibitory concentration tests, and finally, complete genome sequencing of these isolates was carried out using a sequencing technique. A total of 73 strains of S. aureus and seven of E. coli were isolated. Spatypes t11521 (23.91%), t701 (17.39%), and t359 (6.52%) were detected in S. aureus isolates; the enterotoxin genes sei, seq, sem, seo, seu, sen, sek, and seg, representing 2.17% of isolates, respectively, and seh (8.69%). The virulence genes hlgA (100%), hlgB (100%), hlgC (100%), lukD (97.82%), lukE (97.82%), lukF-PV and lukS-PV (4.34% ) both, and the tst gene (2.17%). Regarding phenotypic resistance, 100% of S. aureus isolates were resistant to at least one antibiotic, 90.41% were resistant to cefotaxime, 100% to penicillin G, 64.38% to erythromycin, 73.97% to 73.97% clindamycin, 68.49% to tetracycline and 5.47% to cefoxitin, these being considered methicillin-resistant S. aureus isolates. The most frequent resistance genes in S. aureus isolates are the blaZ (53.57%) and tet(38) (80.35%) genes. The mecA gene was detected in 4.44% and the msrA gene in 4.44% of the isolates. Furthermore, seven strains of E. coli were isolated, considered non-pathogenic, in which the virulence genes csgA (100%), fdeC (66.66%), fimH (66.66%), gad (66.66%), hlyE (100%), iss (100%), lpfA (33.33%), terC (100%), sitA (66.66%), yehA (66.66%), yehB (66.66 %), YehC (100%) and yehD (100%) were detected. Furthermore, 100% of these isolates were resistant to at least one antimicrobial agent, with resistance to gentamicin (57.14%) and ciprofloxacin 85.81% being observed most frequently. The blaEC resistance gene was the only one detected in these isolates. Therefore, the presence of strains resistant to antibiotics detected in artisanal coalho cheese sold in Recife represents a health risk because this might represent another environment for the dissemination of antibiotic resistance. 

COMMITTEE MEMBERS:
Presidente - TATIANA SOUZA PORTO
Interno - JOSE WILTON PINHEIRO JUNIOR
Externo ao Programa - 1756652 - RODRIGO BARBOSA ACIOLI DE OLIVEIRA - UFRPEExterno à Instituição - JOSÉ GIVANILDO DA SILVA - UFBA
Externo à Instituição - MARCELO MENDONCA