Banca de DEFESA: ANDRESSA RAPHAELY DE LIMA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDRESSA RAPHAELY DE LIMA SILVA
DATE: 23/02/2024
TIME: 09:00
LOCAL: Online via Google Meet
TITLE:

Evaluation of potential protective effects of essential oils against ethanol-induced toxic effects in a fetal alcohol spectrum disorder (FASD) model in zebrafish (Danio rerio)

KEY WORDS:

Teratology; early development; phytotherapy; behavioral analysis device; didactic model.

PAGES: 150
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Fetal alcohol spectrum disorder (FASD) is the most common possible cause of preventable intellectual and congenital disabilities yet remains without established treatments. The study aimed to evaluate the protective effects of essential oils (EOs) from Syzygium aromaticum (SEO), Thymus vulgaris (TEO), Origanum vulgare (OEO), and Carum carvi (CEO) and their binary mixtures against the toxic effects of embryonic ethanol (EtOH) exposure during early development in a FASD zebrafish (Danio rerio) model. For this, co-exposure to EtOH (150 mM) and EOs and their mixtures (0.5-1 mg/L) was performed for 6 or 22 hours. Response variables were epiboly at 8 hpf (hours post-fertilization), mortality at 24 hpf, overall development at 24 hpf, and hatching rate at 24 to 96 hpf. An invention patent (IP) for a device for behavioral analyses in zebrafish, a utility model patent (UM) for a three-dimensional teaching model of zebrafish with FASD phenotype, and an industrial design (ID) for three-dimensional teaching models of zebrafish under normal conditions and with FASD phenotype were also developed. As expected, the DMSO used for EOs dissolution did not alter epiboly (74.51% ± 5.22). EtOH delayed epiboly (59.11% ± 6.68). Only SEO + TEO showed complete protection (69.41% ± 6.82) against the toxic effects of EtOH on epiboly, while their isolated OEs provided partial protection. Therefore, SEO, TEO, and SEO + TEO were selected for further analyses. There were no changes in mortality rate at 24 hpf, which remained ≤ 10% in all experimental groups analyzed. For the evaluation of overall development at 24 hpf, 7 stages (S1, S2, S3, S4, S5, S6, and S7) were established based on periods with intervals between 10-24 hpf of zebrafish. For analysis, the stage with the highest percentage of animals per group was considered, with groups predominantly classified in S7 being considered to have preserved development. DMSO did not alter development at 24 hpf (100% at E7). EtOH delayed development at 24 hpf (62.86% at E2). SEO (68.57% at E4), TEO (62.86% at E3), and SEO + TEO (60% at E4) showed partial protection against the toxic effects of EtOH on development at 24 hpf, with SEO and SEO + TEO being more efficient. Regarding the hatching rate, DMSO did not alter hatching (92.75% ± 2.52). EtOH delayed hatching (13.37% ± 0.89). SEO (45.89% ± 2.90), TEO (47.61% ± 4.64), and SEO + TEO (48.36% ± 8.70) showed partial protection against the toxic effects of EtOH on hatching, with no significant differences among them. The PI, MU, and DI were deposited/registered at the National Institute of Industrial Property. It is concluded that zebrafish is suitable for studies involving the analysis of toxic effects of embryonic exposure to EtOH, and that SEO and TEO can be used as therapeutic agents with partial protection, with SEO + TEO being more efficient. Additionally, it is expected that with the IP, contributions will be made to the development of research, and with the UM and ID, to translational science and raising awareness of the topic.

COMMITTEE MEMBERS:
Interno - PABYTON GONCALVES CADENA
Externo à Instituição - EDUARDO PACHECO RICO - UNESC
Externo à Instituição - YURI MATEUS LIMA DE ALBUQUERQUE