Banca de DEFESA: ORION PEDRO DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ORION PEDRO DA SILVA
DATE: 28/02/2024
TIME: 08:30
LOCAL: https://meet.google.com/pii-mtwr-sra
TITLE:

Phenotypic and genotypic resistance profile of Staphylococcus aureus and Escherichia coli isolated from chicken, cattle and pork samples in the state of Pernambuco, Brazil.

KEY WORDS:

Bacterial multidrug resistance, fresh meat, poultry

PAGES: 88
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The objective of this research was to study the profile of phenotypic and genotypic resistance to antimicrobials in isolates of Staphylococcus aureus from commercial broiler and layer chickens, as well as Escherichia coli and S. aureus in meat samples (beef, pork and chicken) “in natura” sold in the city of Recife, Pernambuco. The study was carried out on 200 samples of cloacal and tracheal swabs collected from 100 broiler chickens and 100 commercial layers in the state of Pernambuco, Brazil. Meat samples were collected in all eight Health Districts of the City of Recife. Meat samples were collected in all eight Health Districts of the City of Recife. 120 samples of “in natura” meat were collected, being (40) beef, (40) pork and (40) chicken in commercial establishments (markets, butchers and open-air markets). Of the 200 swab samples analyzed, after isolation and molecular testing with Polymerase Chain Reaction (PCR), 5/200 (2.5%) samples were identified as S. aureus, all coming from commercial layers. Of these, 100% (5/5) presented the norA and norC genes, 3/5 (60%) presented the msrA gene, 1/5 (20%) presented the blaZ gene and 1 (20%) presented the mecC gene. In meat samples, 150 colonies of the genus Staphylococcus were isolated and, after phenotypic and molecular analysis, 10 isolates of S. aureus were identified, of which 7/10 (70%) came from pork and 3/10 (30%) from beef. of chicken and all isolates were multiresistant. Of the isolates, 10/10 (100%) presented the norC gene, 9/10 (90%) presented tet 38 and 6/10 (60%) and the norA and blaZ genes. In the PFGE technique, 8 different pulse types were detected, 2 of which had a similarity of 100; the first clone was present in 2 pork samples and the second clone was present in both pork and chicken meat. In the 40 Escherichia coli isolates, it was observed that 23/40 (57.5%) showed phenotypic resistance to extended-spectrum beta-lactams. In the genotypic tests, 23/40 (57.5%) presented the blaTEM gene and 21/40 (52.5%) the blaSHV gene. It is concluded that there is a great need for intervention and health education with guidance to veterinarians and producers regarding the rational use of antibiotics, making intervention by human and animal health bodies necessary to combat antimicrobial resistance.

COMMITTEE MEMBERS:
Presidente - JOSE WILTON PINHEIRO JUNIOR
Externa ao Programa - 3309515 - ANDREA PAIVA BOTELHO LAPENDA DE MOURA - UFRPEExterna ao Programa - 385042 - MARIA JOSE DE SENA - UFRPEExterno à Instituição - JOSÉ GIVANILDO DA SILVA
Externa à Instituição - SINEIDE MARIA DE OLIVEIRA VILELA