Despite recent advances in the diagnosis and treatment of Canine Leishmaniasis, the disease remains a major veterinary challenge. Despite the variety of clinical manifestations, most infected animals develop dermatological signs. Therefore, the treatment of ulcerated skin lesions in dogs with leishmaniasis often presents difficult clinical resolution due to secondary bacterial infection. Therefore, this research aimed to isolate and identify the microbiota present in ulcerated lesions through microbial culture, and evaluate the diversity of the microbial community through metagenomics. For this purpose, 20 animals of both sexes and without defined breed with a positive diagnosis through the parasitological and serological technique for Canine Leishmaniasis were used. Initially, samples were collected using sterile swabs from open, non-purulent skin wounds, after which microbiological culture was carried out using EMB Agar and 5% Sheep Blood Agar media for bacterial isolation and preliminary identification using Gram staining. The results showed the presence of 13 Staphylococcus isolates; 10 isolates of Coagulase Negative Staphylococci; seven isolates of non-glucose-fermenting Gram-negative bacilli; five isolates of Proteus spp.; four isolates of alpha hemolytic Streptococcus; four isolates of beta hemolytic Streptococcus; three isolates of Streptococcus spp. and Pseudomonas aeruginosa; two isolates of Corynebacterium spp, group D Streptococcus, non-Enterococcus, Klebsiella spp.; an isolate of Enterococcus spp., Escherichia coli and Micrococcus spp. Although the isolated microorganisms are part of the commensal microbiota of dogs' skin, it is observed that knowledge of these microorganisms can lead to more assertive therapy, as skin lesions caused by Leishmaniasis may suffer some interference in the healing process due to the presence of these microorganisms. bacteria.