Banca de QUALIFICAÇÃO: ORION PEDRO DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ORION PEDRO DA SILVA
DATE: 28/09/2023
TIME: 16:30
LOCAL: https://meet.google.com/ugg-iiiq-ind
TITLE:

Profile of phenotypic and genotypic resistance of Staphylococcus aureus in commercial broilers and laying hens, Staphylococcus aureus and Escherichia coli in beef, pork and chicken in Pernambuco.

KEY WORDS:

Bacterial multidrug resistance, fresh meat, poultry

PAGES: 55
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The objective of this research was to study the profile of resistance to antimicrobials in isolates of Staphylococcus aureus from broilers and laying hens, and Escherichia coli and S. aureus in meat samples (beef, pork and broilers) in natura consumed in the municipality of Recife PE. To verify the genotypic resistance profile of S. aureus in commercial broilers and laying hens in the state of Pernambuco. verify the antimicrobial resistance profile, identify multiresistant strains; Detect the presence of resistance genes (blaZ, mecA, mecC, norA, norC, tet 38, msrA, blaTEM and blaSHV, genetically type using PFGE and MLST techniques, analyze the genetic sequences. The study was carried out from 200 samples of cloacal and tracheal swabs collected from commercial broilers and laying hens in the state of Pernambuco, Meat samples were collected in all 8 sanitary districts of the city of Recife. Where 120 samples were collected, (40) from beef, (40) pork and (40) fresh chicken, in commercial establishments (markets, butchers and free fairs) located close to SUS primary care units. Of the 200 swab samples, after molecular tests with PCR, 5 samples were genotypically identified as S. aureus, of which 5/5 (100%) presented the norA and norC genes, 3/5 (60%) presented the msrA gene, 1 (20%) presented the blaZ gene and 1 (20 %) presented the mecC gene. From the meat samples, 150 (one hundred and fifty) suspicious colonies were isolated, which after biochemical tests, morphotinctorial analysis and molecular analysis (PCR), 10 (ten) positive colonies for S. aureus were isolated, of which 7 (seven) were pork and 3 (three) chicken and none of beef. In the phenotypic resistance tests, all colonies were considered multiresistant, as they showed resistance to more than 3 classes of antibiotics. Of the 10 resistant isolates 10/10 (100%) harbored the norC gene, 9/10 (90%) harbored tet 38, and 6/10 (60%) harbored both the norA and blaZ genes. In tests to identify E. coli, 42 phenotypic and biochemically compatible colonies were isolated, which were phenotypically tested for resistance to extended-spectrum beta-lactams, where 23/42 (53%) showed phenotypic resistance and 21/42 (47%) did not show resistance, while in the genotypic tests 23/42 (43%) showed the blaTEM gene, 21/42 (40%) the blaSHV gene, and only 9/42 (17%) did not show any tested gene. With the isolation and identification of strains of S. aureus and E. coli identified as multiresistant, both genotypically and phenotypically, we emphasize the real need for intervention and health education with instructions to the population and producers regarding the rational use of antibiotics. We warn of the need for joint intervention by health bodies, and dissemination in the media to face this problem that puts human, animal and environmental health at risk.

COMMITTEE MEMBERS:
Presidente - RINALDO APARECIDO MOTA
Interno - JOSE WILTON PINHEIRO JUNIOR
Externa ao Programa - 3309515 - ANDREA PAIVA BOTELHO LAPENDA DE MOURA - UFRPE