Banca de DEFESA: CLÁUDIO BARBOZA DE ANDRADE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CLÁUDIO BARBOZA DE ANDRADE
DATE: 31/07/2023
TIME: 14:30
LOCAL: Videocanferência - https://meet.google.com/pts-qjci-wnz
TITLE:

EVALUATION OF THE HEALING EFFECT OF OINTMENT CONTAINING ESSENTIAL OIL OF Cananga odorata IN EXCISIONAL CUTANEOUS LESIONS OF WISTAR RATS

KEY WORDS:

Ylang-Ylang; Phytotherapy; Phytochemistry; Microbiology; Histology.

PAGES: 65
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SUMMARY:

Cananga odorata, a tree originally from Asia, has essential oil (EO) obtained from its flowers with various properties known as anti-inflammatory, antimicrobial, and antioxidant. The aim of this study was to analyze the phytochemical profile, antimicrobial activity, and wound healing effect of C. odorata EO on skin lesions in rats. The EO was obtained commercially and analyzed using the gas chromatography-mass spectroscopy method. The microbiological analysis was evaluated using the minimum inhibitory concentration test and determination of  the minimum bactericidal concentration. The ointment formulation consisted of 10% C. odorata EO incorporated into solid vaseline, followed by a microbiological evaluation to exclude the presence of contaminants. The ointment was tested on excisional dorsal skin wounds, surgically made under dissociative anesthesia, in male Wistar Albino rats (n=60), divided into three groups (n=20): absolute control group (untreated wounds); control group (vaseline only); and treated group (OE-based ointment) with topical administration once a day. The skin wounds were evaluated in each subdivision of groups (n=5) corresponding to days 3, 7, 14, and 21 after each treatment.  Macroscopic analyses of the lesions were estimated by the degree of wound contraction. Lesion sections fixed in formalin and processed histologically were used for histomorphological analyses.  Phytochemical analysis identified 28 components, with β-caryofinel being the most abundant (19%). Microbiological analysis of the ointment showed no contaminants, as well as the antimicrobial activity of EO against the tested microorganisms (Listeria monocytogenes, Salmonella enterica Sor. Typhimurium, S. aureus, S. aureus ATCC 25923). Although the wound healing effect of the formulated ointment was not statistically significant (p > 0.05), it led to less crust formation and reduced inflammation in the lesions. In conclusion, the commercial  C. odorata EO presented variations in the phytochemical composition when compared to previous studies. The microbiological results reinforced the antimicrobial activity of C. odorata EO, including against  skin-inherent microorganisms. The wound healing effect, although not statistically significant, showed reduced inflammation.

COMMITTEE MEMBERS:
Presidente - JOAQUIM EVENCIO NETO
Externo à Instituição - JOSE DE CASTRO SOUZA NETO JUNIOR - UFPE
Externa à Instituição - LIGIA REIS DE MOURA ESTEVÃO