Banca de DEFESA: ÉRIQUE RICARDO ALVES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ÉRIQUE RICARDO ALVES
DATE: 28/02/2023
TIME: 14:00
LOCAL: Recife - PE
TITLE:

INVESTIGATION OF THE MECHANISMS OF ACTION OF MELATONIN, ASSOCIATED OR NOT WITH QUERCETIN ON THE LIVER AND KIDNEYS OF DIABETIC RATS AT THE CELLULAR, BIOCHEMICAL AND IMMUNOHISTOCHEMICAL LEVEL.

KEY WORDS:

hyperglycemia, melatonin, flavonoid, oxidative stress, inflammation, rats.

PAGES: 104
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Diabetes mellitus is a chronic hyperglycemic disease, its complications affect all organs of the body, including the liver and kidneys, and it is already known that oxidative stress and inflammation are part of its pathophysiology. In this sense, two antioxidants stand out: quercetin and melatonin, both also with anti-inflammatory potential. In this sense, the present work aimed to analyze the effect of treatment with quercetin associated or not with melatonin in the kidneys and liver of diabetic rats. Ninety male rats divided into the following groups were used: Group I (Control): rats without induced diabetes; Group II: diabetic rats (GD); Group III: diabetic rats treated with insulin (GDI); Group IV: diabetic rats treated with melatonin (GDM); Group V: diabetic rats treated with quercetin (GDQ); Group VI: diabetic rats treated with melatonin and quercetin (GDMQ). Diabetes was induced by streptozotocin (60 mg/kg). Melatonin was administered (10 mg/kg) for 30 days after diabetes induction. Quercetin was administered (40 mg/kg) also for 30 days after diabetes induction. Glucose was measured using strips and a commercial glucometer. At the end of the experiment, the animals were anesthetized and euthanized, the organs were collected and the blood was centrifuged and the serum was saved for analysis. The organs were sent for routine histological processing (H.E slides for histopathology and morphometry) and the serum was sent for biochemical analysis for creatinine, urea, TGO and TGP. Tissue oxidative stress was also performed to assess lipid peroxidation and reduced glutathione. In addition, immunohistochemistry was performed for IL-6, TNF-α and Tunnel Test (apoptosis) in the kidneys and IL-6, TNF-α and IL-10 for the liver. The results showed that treatments with melatonin alone and melatonin + quercetin were more effective in lowering glycemic levels, protecting animals against body and kidney weight loss, in addition to reducing oxidative stress and avoiding severe pathological changes in the kidneys, as well as protecting the renal tissue and stabilize levels of creatinine and urea, in addition they protected against the increase of apoptosis and inflammatory interleukins. Treatment with quercetin alone showed mild or moderate effectiveness in the aggravations of this disease. In the liver, the groups treated with melatonin and quercetin, both together and separately, managed to protect the animals against hepatic weight loss, in addition to reducing oxidative stress and avoiding severe pathological changes in the liver. Furthermore, it stabilized the serum levels of TGO and TGP, and prevented the increase of inflammatory interleukins (IL-6 and TNF-α) and reduction of IL-10 (anti-inflammatory interleukin). It is concluded that for the renal tissue, treatments with melatonin alone and melatonin associated with quercetin were the most effective against renal complications in diabetes. However, for the liver, the same treatments associated or not, were beneficial in protecting against the damage caused by diabetes.

 

COMMITTEE MEMBERS:
Presidente - VALERIA WANDERLEY TEIXEIRA
Interno - ALVARO AGUIAR COELHO TEIXEIRA
Externo ao Programa - 1929164 - FRANCISCO DE ASSIS LEITE SOUZA - UFRPEExterna à Instituição - ISMAELA MARIA FERREIRA DE MELO
Externo à Instituição - LEUCIO DUARTE VIEIRA FILHO - UFPE