Banca de DEFESA: TATIENE ROSSANA MÓTA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : TATIENE ROSSANA MÓTA SILVA
DATE: 28/06/2022
TIME: 10:00
LOCAL: https://meet.google.com/vjb-ibov-yti
TITLE:

Entomological survey and natural infection by Trypanosoma cruzi in triatomines in a Agreste region of Pernambuco, Brazil

KEY WORDS:

Vectors, Chagas disease, DTUs, trypanosomatids

PAGES: 117
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Trypanosoma cruzi presents a heterogeneous population with seven lineages recognized, called Discrete Typing Units (DTU's). The knowledge of T. cruzi DTUs can contribute to a better understanding of the epidemiology of Chagas Disease and the possible risk factors for infection to man. The aim of this study was to carry out an entomological survey of triatomines, detect the frequency of T. cruzi infection and identify the lineages present in municipalities in the rural region of Pernambuco, Brazil. Based on data from the the Brazilian National Chagas Disease Control Program (PNCDCh), a retrospective study (2008-2017) was carried out to assess the occurrence and distribution of triatomines in 21 municipalities. In addition, during the period from July 2018 to June 2019, triatomines were collected in intradomicile and peridomicile area and identified for subsequent DNA extraction. The search for T. cruzi was carried out through parasitological and molecular exams. For the DNA research of Trypanosoma spp. PCR was performed, the amplified products were purified and sequenced. T. cruzi genotyping was performed by Multiplex PCR. To evaluate the spatiotemporal distribution of triatomines infected by trypanosomatid parasites, data from the PNCDch (2008-2018) were used, the total number of triatomines captured from intradomicile and peridomicile areas, as well as the infection rate (IR) by trypanosomatid. The method by Getis–Ord method was used to statistically identify significant concentration clusters and the IR of triatomines by trypanosomatids. A generalized linear regression model with a binomial distribution was used to evaluate the probability of of finding an IR by trypanosomatids. Through the retrospective study, a total of 4694 triatomines were collected, 94.5% and 5.5% from the intradomiciliary and peridomiciliary environments. The most frequent species were Panstrongylus lutzi (30.36%), Triatoma brasiliensis (26.12%), Triatoma pseudomaculata (22.43%) and Panstrongylus megistus (20.54%). A total of 117 triatomines were collected, 93.16% and 6.84% from the intradomiciliary and peridomiciliary environments, respectively. The captured species were P. lutzi (37.60%), T. pseudomaculata (26.50%), T. brasiliensis (23.08%) and P. megistus (12.82%). The T. cruzi infection rate was 5.49% and 12.09% in parasitological and molecular tests, respectively. The analysis molecular detected 70.59% of the TcI genotype in all triatomine species identified and 29.41% of the TcIII/TcIV in P. megistus and P. lutzi. The analysis of the spatiotemporal distribution of triatomines infected by trypanosomatids was performed with 4,800 triatomines, flagellar forms similar to T. cruzi were detected in 10.29% of them, the majority of positive specimens (98.17%) were collected at intradomicile. The geospatial analyses identified triatomines clusters in intradomicile and peridomicile environments. The logistic regression for species (P. lutzi, P. megistus, T. brasiliensis and T. pseudomaculata) showed that the probability of detection of T. cruzi infection remains constant in up to 50 specimens examined or more. The presence of infected triatomines in intra and peridomicile areas calls for the implementation of surveillance actions, such as health education in areas where these vectors are most vulnerable. In addition, adoption of systematic entomological control measures to reduce the risks of infestation and, consequently, of Chagas disease.

BANKING MEMBERS:
Presidente - 1680404 - GILCIA APARECIDA DE CARVALHO
Interno - 384005 - LEUCIO CAMARA ALVES
Externo à Instituição - CARLOS ALBERTO DO NASCIMENTO RAMOS - UFMS
Externa à Instituição - VALÍRIA CERQUEIRA DUARTE - UFPA
Externo à Instituição - EDYNIESKY FERRER MIRANDA - UFPE