Banca de DEFESA: WELLINGTON LEAL DOS SANTOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : WELLINGTON LEAL DOS SANTOS
DATE: 24/02/2022
TIME: 08:30
LOCAL: Virtual Google Meet
TITLE:

Bioprospecting of bioactive molecules from fava bean (Phaseolus lunatus L.): multifunctionality and applications

KEY WORDS:

Bioaccessibility; bioproducts; lima beans; Potential antioxidant.

PAGES: 142
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The use of products of agricultural origin, including legume seeds, for the development of functional and biological ingredients, can be an economic alternative for the food and pharmaceutical industry. Thus, the objective was to characterize the functional and nutritional aspects and the infrared spectra of the flours obtained from the varieties, Rosinha (V1), Olho de Ovelha (V2), Galo de Campina (V3), Mulatinho (V4) , Branquinha (V5), Lavandeira (V6) of P. lunatus L. for use in food matrices, in addition to the effect of gastrointestinal digestion on the antioxidant potential of proteins, hydrolysates and phenolic compounds present in fava beans. The presence of anti-nutritional factors, water absorption capacity (CAA) and oil (CRO), water solubility (ISA), foaming capacity (CFE), emulsifier (EC), foam stability (EFE) were evaluated. , flour emulsion (EE) and flour infrared spectra. Subsequently, the flours were subjected to extraction of phenolic compounds using hexane (E1), petroleum ether (E2), chloroform (E3), ethyl acetate (E4), ethanol (E5), acetone (E6) and water (E7) in the proportion 1:5 (m/v). The phenolic compounds obtained were submitted to in vitro gastrointestinal digestion and to the determination of the antioxidant potential against the DPPH, ABTS radical and copper and iron chelation. Finally, the flours of the Lavandeira, Rosinha, Olho de Ovelha and Mulatinho varieties were subjected to alkaline solubilization protein extraction, followed by isoelectric precipitation, later, the samples were submitted to the in vitro gastric and intestinal digestion test, and soon after, the antioxidant tests against radical scavenging assays (DPPH), (ABTS), hydroxyl, superoxide, ferric reducing power (FRAP), reducing power, anti-protein denaturation activity, inhibition of lipid peroxidation, survival of Saccharomyces cerevisiae against oxidative stress and copper and iron chelation were performed. The flours showed low levels of trypsin inhibitors (2.21 - 2.24 ITU.g-1) and high levels of amylase inhibitors (4.68 - 13.52 ITU.g-1), in addition, CAA ( 149 - 240 gg-1), ISA (2.54 -2.83 gg-1), CAA (0.63 - 102 gg-1), CE and EE remained above 50%, with stability after 60 minutes. Peaks were observed in the spectral ranges between 500 and 3350 cm-1 with stretch regions in OH, CH, aromatic rings, amide I and amide II. Water was more efficient in extracting phenolic compounds in varieties V1, V3, V4, V5, V6 and petroleum ether for V2 before the digestive process. Gastrointestinal digestion resulted in an increase in the phenolic compounds present in the extracts and against the scavenging of DPPH, ABTS and iron and copper chelation, in relation to the protein fraction, in vitro digestion proved to be an effective tool to obtain protein hydrolysates with antioxidant capacity against the elimination of ABTS, DPPH, hydroxyl, superoxide radicals and chelation of copper and iron. In addition, anti-denaturing effects, decreased lipid peroxidation and survival of S. cerevisiae were observed. The results of the present study suggest that flours from the P. lunatus L. varieties could be used as a potential substitute in the formulation of food products with high technological, nutritional and biological value.

BANKING MEMBERS:
Interna - 1508562 - KEILA APARECIDA MOREIRA
Externa à Instituição - DANIELE SILVA RIBEIRO
Externo à Instituição - JOSÉ ERICK GALINDO GOMES
Externa à Instituição - ROSANGELA ESTEVAO ALVES FALCAO
Externa à Instituição - TALITA CAMILA EVARISTO DA SILVA NASCIMENTO