Hematological parameters, serum biochemistry, blood cell ultrastructure and defensive behavior of Leptodactylus vastus lutz, 1930 in Atlantic forest fragments of northeastern Brazil
ecophysiology, hematology, anuran, antipredatory behavior
The environmental impacts caused by anthropic action and nature's actions have alerted to the loss of anuran biodiversity. Thus conservation biology, conservation medicine, and ecology have presented results on intrinsic and extrinsic factors aimed at evaluating the physiological state of anurans. Among the intrinsic parameters, we can highlight the analysis of the blood count, serum biochemistry, as well as the investigation of the occurrence of blood parasites while the description of the defensive repertoire has been considered as an extrinsic factor for the investigation of antipredatory behavioral displays presented by anurans. In this way, the objective of this PhD thesis was to evaluate the physiological state of males Leptodactylus vastus Lutz, 1930 in fragments of Semideciduous Atlantic Forest in northeastern Brazil. For the analysis of intrinsic factors 30 males of L. vastus were collected in the Botanical Garden and Dois Irmãos State Park. To describe the defensive repertoire 30 males were captured in the Mata do Camocim Conservation Unit located in the municipality of São Lourenço da Mata. The three areas are located in the metropolitan region of Recife. Males of L. vastus had an average of hematological and serum biochemical variables close to those reported for other anuran species. However, high hematocrit values may suggest dehydration/hemoconcentration problems, as well as increased basophil values and abnormalities in erythrocytes may suggest an immunological hypersensitivity in the specimens. In addition, enzyme values for liver functions suggest possible damage to the organ, but further diagnostic tests are necessary. Regarding defensive behaviors, males exhibited five types of behavior divided into visual mechanism: puffing up the body (100%), push movement (83.3%), hiding (23.3%), cloacal discharge (16.6%) and spine aggression (6.6%); and acoustic mechanism: distress call (33.3%). Thus analysis of anuran ecophysiology has been shown to be an important tool to assist in monitoring the physiological state of anurans, and in the creation of strategies for the conservation of these animals and the ecosystems associated with them.