Banca de DEFESA: PERICLES TAVARES AUSTREGESILO FILHO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : PERICLES TAVARES AUSTREGESILO FILHO
DATE: 06/02/2024
TIME: 13:30
LOCAL: meet.google.com/jvz-ukqb-qti
TITLE:

Phytochemical and antimicrobial activity evaluation of Melaleuca leucadendra (Myrtaceae) extract on Staphylococcus aureus isolated from bovine mastitis

 

KEY WORDS:

Phytotherapy; Mastitis; Metabolites; Antimicrobial Resistance

PAGES: 94
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

The aim of this study was to carry out the phytochemical evaluation and antimicrobial activity of the hydroalcoholic and hexane extract of Melaleuca leucadendra leaves (Myrtaceae), at a concentration of 20mg/ml, on Staphylococcus aureus isolated from bovine mastitis. After identifying the plant, the extracts were prepared and submitted to phytochemical and antibacterial tests. Verification of the presence of metabolite classes was performed by chromatographic analysis using traditional techniques and verification of compounds by mass spectrometry coupled to electrosplay HPLC-DAD-ESI(+)-MS/MS (microTOF-QII) (ESI-MS) ; the hydroalcoholic and hexane extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). To evaluate the antibacterial action, the inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were verified in isolates of Staphylococcus aureus from bovine mastitis. The presence of terpenes, glycosylated flavonoids, aglycone flavonoids, triterpenes, tannins was verified and spectrometry identified 13 chemical superclasses with 88 compounds. In the GC-MS chromatogram, 37 peaks were found, corresponding to 37 chemical compounds. Hydroalcoholic and hexane extracts showed antimicrobial activity against Staphylococcus aureus strains that cause bovine mastitis, proving to be an alternative in the treatment of the disease.

COMMITTEE MEMBERS:
Presidente - JOSE WILTON PINHEIRO JUNIOR
Externo à Instituição - René Duarte Martins
Externa à Instituição - EULINA TEREZA NERY FARIAS
Externo à Instituição - GIBSON GOMES DE OLIVEIRA
Externo à Instituição - JOSE ANTONIO DE SOUSA PEREIRA JR