Banca de QUALIFICAÇÃO: ANDRESSA RAPHAELY DE LIMA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDRESSA RAPHAELY DE LIMA SILVA
DATE: 21/12/2023
TIME: 08:00
LOCAL: Online via Google Meet
TITLE:

Evaluation of possible protective effects of essential oils against ethanol-induced toxic effects in a fetal alcohol spectrum disorder (FASD) model in zebrafish (Danio rerio)

KEY WORDS:

Teratology; early development; phytotherapeutic; behavioral analysis device; didactic model

PAGES: 144
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

The fetal alcohol spectrum disorder (FASD) encompasses all effects associated with prenatal alcohol exposure, considered a possible leading cause of preventable intellectual and congenital disabilities. Despite this, there are still no established treatments for its prevention or reversal of its effects, emphasizing the importance of continued exploration of therapeutic alternatives. The aim of this study was to evaluate the protective effects of essential oils (EOs) from clove (COE), white thyme (WTE), oregano (OEO), and caraway (CAE), as well as their binary mixtures, against the toxic effects of embryonic exposure to ethanol (EtOH) during early development in a FASD model using zebrafish (Danio rerio). Co-exposure to EtOH (150 mM) and EOs and their mixtures (0.5-1 mg/L) was conducted for 6 or 22 hours. Response variables included epiboly at 8 hpf (hours post-fertilization), mortality at 24 hpf, overall development at 24 hpf, and hatching rate at 24 to 96 hpf. Additionally, a utility model (UM) patent for a three-dimensional didactic model of zebrafish with a FASD phenotype and an industrial design (ID) patent for three-dimensional didactic models of zebrafish in normal and FASD phenotypes were developed. As expected, the DMSO used for EO dissolution did not alter epiboly (74.51% ± 5.22), while EtOH delayed epiboly (59.11% ± 6.68). Regarding the protection of EOs against EtOH toxic effects on epiboly, only COE + WTE showed complete protective effects (69.41% ± 6.82), with their isolated oils providing partial protection. Therefore, COE, WTE, and COE + WTE were selected for further analysis. DMSO, EtOH, COE, WTE, and COE + WTE did not alter the mortality rate at 24 hpf, remaining ≤ 10% in all experimental groups analyzed. For overall development at 24 hpf, 7 stages (E1, E2, E3, E4, E5, E6, and E7) were established based on intervals between 10-24 hpf of zebrafish. The stage with the highest percentage of animals per group was considered for analysis, with groups predominantly classified as E7 indicating preserved development. DMSO did not alter development at 24 hpf (100% at E7). EtOH delayed development at 24 hpf (62.86% at E2). COE (68.57% at E4), WTE (62.86% at E3), and COE + WTE (60% at E4) showed only partial protection against EtOH toxic effects on overall development at 24 hpf, with COE and COE + WTE being more effective than WTE. Regarding the hatching rate, DMSO did not alter hatching (92.75% ± 2.52). EtOH delayed hatching (13.37% ± 0.89). COE (45.89% ± 2.90), WTE (47.61% ± 4.64), and COE + WTE (48.36% ± 8.70) provided only partial protection against EtOH toxic effects on hatching, with no significant differences between them. The patent, utility model, and industrial design were successfully developed, all deposited with the National Institute of Industrial Property (INPI). In conclusion, zebrafish is suitable for studies involving the analysis of toxic effects of embryonic EtOH exposure during early development, as it exhibited alterations in the early stages of development without affecting the mortality rate. COE and WTE showed partial protective effects against toxic effects of embryonic EtOH exposure during early development, with COE + WTE being more efficient, indicating possible synergistic activity between these EOs. Further studies investigating the interaction between these EOs and their protection against toxic effects of embryonic EtOH exposure are necessary. Additionally, with the patent deposit, it is expected to contribute to the development of research conducting behavioral analyses of zebrafish and related species. With the utility model deposit and industrial design registration, it is hoped to assist in understanding the effects that alcohol can trigger in development, contributing to translational science and awareness on the subject.

COMMITTEE MEMBERS:
Externo à Instituição - EDUARDO PACHECO RICO
Externa à Instituição - MARIANE CAJUBÁ DE BRITTO LIRA NOGUEIRA - UFPE