Banca de DEFESA: MAURÍCIO DA ROCHA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MAURÍCIO DA ROCHA COSTA
DATE: 23/02/2023
TIME: 14:00
LOCAL: Virtual Google Meet
TITLE:

Evaluation of the antimicrobial activity of an oral antiseptic based on Melon-of-São-Caetano (Momordica charantia) on pathogenic microorganisms of the oral cavity

KEY WORDS:

Antimicrobial Agents; Pasteurella multocida; Candidiasis; Antiobiotics.

PAGES: 46
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Momordica charantia (Melon-de-São-Caetano) is a plant belonging to the Cucurbitaceae family, widely distributed in tropical and subtropical areas around the world, mainly in Asia, India, China and Brazil, where it is traditionally used as a medicinal plant, and the fruits of some varieties of M. charantia are consumed as food. In addition, Melon-de-São-Caetano is a plant species that has attracted the interest of researchers in recent years, studies have determined that this plant contains a wide range of bioactive compounds with therapeutic potential such as charantine, α-momorcharina and MAP30, highlighting its properties as antidiabetic, antiulcer, antioxidant, antihyperglycemic and antimicrobial. Thus, this work aimed to develop and evaluate in vitro an antiseptic bioproduct for the control of microorganisms present in common oral diseases in companion animals. For this, fruits, leaves and seeds of M. charantia were collected, the cleaning and drying process was carried out and the material was ground separately. From there, the plant material was macerated with 99.5% ethanol, filtered and then rotary evaporated, leaving only the crude ethanolic extract. This material was diluted and separated into concentrations of 2 mg/mL, 1 mg/mL, 0.5 mg/mL, 0.25 mg/mL, 0.125 mg/mL and 0.062 mg/mL. With these fractions, the Minimum Inhibitory Concentration (MIC) assays were performed with Pasteurella multocida, Candida albicans, Candida tropicalis and Candida Famata. Based on the MIC value, a mouthwash was produced, with the active ingredient extract of the leaf, fruit and seed, separately. Although some studies question the antibacterial activity of gram-negative bacteria from M. charantia, in this study, the antimicrobial activity of the ethanolic extract of the leaf and seed of M. charantia from 1 mg/mL of the leaves and 0 ,25 mg/mL and the seed that were able to inhibit the growth of P. multocida, this result was both for the MIC from the extract and the rinse, according to studies, flavonoids may be the compounds responsible for this activity antimicrobial activity, both the extract and the mouthwash were also able to inhibit candida growth. Thus, it is envisioned that the mouthwash based on M. charantia can have great efficiency as an antimicrobial, being a phytotherapeutic potential for the control of microorganisms in the oral cavity, such as C. albicans, C. tropicalis, C. chamata and P. multocida, and studies are needed to determine the appropriate and safe dosage.

COMMITTEE MEMBERS:
Presidente - KEILA APARECIDA MOREIRA
Externo ao Programa - 1885755 - PEDRO GREGORIO VIEIRA AQUINO - UFRPEExterna à Instituição - ROSANGELA ESTEVAO ALVES FALCAO