Banca de DEFESA: MARIA VANESSA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA VANESSA DA SILVA
DATE: 25/02/2022
TIME: 09:00
LOCAL: Recife - PE
TITLE:

EVALUATION OF THE EFFECTS OF MELATONIN ON REPRODUCTIVE ASPECTS OF WISTAR RATS SUBMITTED TO CHRONIC ALCOHOL CONSUMPTION

KEY WORDS:

Pineal; placental development; rats; alcohol; antioxidant; oxidative stress.

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Chronic alcohol consumption is a risk factor for maternal and fetal health during pregnancy. Data indicate a prevalence rate of 9.8% in the world population. From this perspective this fact can lead to fetal alcohol syndrome and increased production of reactive oxygen species leading to fetal disorders. On the other hand, the placenta produces a variety of cytokines that when regulated are essential for a healthy pregnancy. However, when unregulated, they can interrupt fetal and placental development pathways, causing congenital deformities and complications. Currently, the protective effect of melatonin against the harm caused by reactive oxygen species and its action on inflammatory cytokines has been verified. Thus, this research aimed to analyze the effects of exogenous administration of melatonin during pregnancy on histomorphometric and immunohistochemical aspects in placentas of rats submitted to chronic alcohol consumption. We used 30 albino rats divided into 3 groups: Control – 10 pregnant rats that did not receive alcohol; Alcohol – 10 pregnant rats submitted to chronic alcohol consumption; Alcohol + Honey - 10 pregnant rats submitted to alcohol consumption associated with melatonin. Alcohol was administered at a dose of 3g/kg by gavage, while melatonin was administered at night at a dose of 0.8mg/kg intraperitoneally. Placentas were weighed and analyzed histopathologically, morphometrically and immunohistochemically. A significant reduction in placental weight was observed in the Alcohol group, while in the Alcohol + Honey group the means were equal to the Control. Histopathological analyzes showed disorganization in the labyrinth zone, while morphometry indicated a reduction in maternal and fetal vessels in the Alcohol group. In the animals of the Control and Alcohol + Honey groups, no significant histological or morphometric alterations were observed. Immunohistochemical analyzes revealed strong marking for the factors TNF α and VEGF in the placentas of females from the alcohol group, when compared to the placentas of females from the control and alcohol + honey groups, which was confirmed by the quantification of these factors. Regarding PCNA staining for cell proliferation, the females of the control group and the alcohol + honey group had the highest percentage of positive cells, significantly differing from the placentas of the females of the alcohol group, which had the lowest percentage. Thus, it is concluded that melatonin has a potential for protective action on the oxidative damage of ethanol in the placenta.

BANKING MEMBERS:
Presidente - 2308550 - VALERIA WANDERLEY TEIXEIRA
Interno - 384942 - ALVARO AGUIAR COELHO TEIXEIRA
Externa à Instituição - ISMAELA MARIA FERREIRA DE MELO