Banca de QUALIFICAÇÃO: JULIANA BERNARDO DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JULIANA BERNARDO DA SILVA
DATE: 08/06/2026
TIME: 09:00
LOCAL: Plataforma Google Meet - https://meet.google.com/ubw-mabx-tno
TITLE:

Prospecting lactic acid bacteria isolated from raw milk: safety assessment and technological characterization

KEY WORDS:

Bioactivity, Microencapsulation, Donkeys, Liposomal systems.

PAGES: 134
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Inspeção de Produtos de Origem Animal
SUMMARY:

Bioactive peptides from donkey colostrum represent a promising alternative to conventional drugs due to their pharmacological properties; however, they are susceptible to enzymatic degradation, which compromises their bioavailability. This study aimed to evaluate the bioactive activities of free and liposome-encapsulated peptides obtained from donkey colostrum, as well as to explore the effects of different cholesterol ratios in liposomal vesicles. The proteins were hydrolyzed by the enzyme trypsin, characterized by degree of hydrolysis and electrophoresis. The hydrolysates were encapsulated in liposomal vesicles composed of phosphatidylcholine and cholesterol (8:2). The findings showed a degree of hydrolysis of 43.78 to 52.99% and an encapsulation rate between 45.00 and 55.69%. In the antioxidant assay with ABTS, higher values were observed among the free peptides (82.51%), and in the DPPH assay, the values of the encapsulated peptides were higher than those of the free peptides. In the β-glucosidase inhibition assay, a decrease in the bioactivity of the encapsulated peptides was observed. In the anti-inflammatory activity test, the encapsulation process also influenced the ability to inhibit protein denaturation. Variable proportions of cholesterol and peptides were tested, characterized by scanning electron microscopy, and tested for antidiabetic and anti-inflammatory activity. The results indicated that formulations with intermediate cholesterol concentrations (1.68 mg) and lower peptide concentrations (0.5 mg/mL) showed greater encapsulation efficiency (~54%) and better morphological organization. Anti-inflammatory activity was significantly higher in liposomes with cholesterol at intermediate concentrations, and antidiabetic activity was superior in formulations with higher concentrations of peptides and cholesterol. It is concluded that bioactive peptides from donkey colostrum, whether free or encapsulated in liposome vesicles, exhibit high therapeutic potential; however, optimizing the ratio between peptides, phospholipids, and cholesterol is fundamental to maximizing encapsulation efficiency and the bioactivity of donkey colostrum peptides, conferring greater stability and potential for pharmacological and nutritional applications.

COMMITTEE MEMBERS:
Presidente - ***.569.911-** - KEILA APARECIDA MOREIRA - UFRPE
Interna - ANA LUCIA FIGUEIREDO PORTO
Externa à Instituição - ELIZABETE RODRIGUES DA SILVA
Externo à Instituição - LEANDRO FRAGOSO LINS