Banca de QUALIFICAÇÃO: ANNA CAROLINA BATISTA E SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANNA CAROLINA BATISTA E SILVA
DATE: 28/11/2025
TIME: 14:00
LOCAL: NUBIOTEC
TITLE:

Development of a powder preparation containing proteases for potential use in veterinary medicine

KEY WORDS:

Fungal proteases; Aspergillus heteromorphus; Lyophilization; Enzyme stability; Veterinary medicine.

PAGES: 72
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Collagenases have broad potential for use in fields such as leather processing, cosmetics, food, and medicine, especially in wound healing. Microorganisms such as filamentous fungi (Aspergillus heteromorphus) are advantageous sources of these enzymes due to their high productivity and extracellular secretion. To improve stability and shelf life, technological advances have been applied, including drying methods such as spray drying and lyophilization, which enable the efficient and economically viable production of enzymatic powder preparations. Thus, this study aimed to produce a preparation containing collagenolytic proteases from A. heteromorphus with potential application in veterinary medicine. A bibliometric analysis was initially conducted to assess trends in the applications of proteases with collagenolytic activity in the field of veterinary medicine. To obtain the enzymatic extract, the microorganism was cultivated using 3 g of wheat bran at 20% moisture, at 30°C for 96 hours. The crude extract containing the A. heteromorphus protease was subjected to pre-purification by precipitation with 70% acetone and subsequent purification by ion-exchange chromatography (DEAE-Sephadex A50) using 0.1 M Tris-HCl buffer, pH 8.0. The stability of the powder and liquid formulations of the purified protease samples was monitored during refrigerated storage for 60 days. The bibliometric analysis showed that collagenase, even when applied indirectly, is a practical, safe, and promising tool, reinforcing the need for further studies in veterinary medicine. The analysis also highlighted the leadership of Asian and European regions in scientific production, driven by greater access to investments and advanced technologies. However, limited interaction among international research groups was observed, indicating that progress in this field also depends on the establishment of global collaboration networks. The protease produced by A. heteromorphus exhibited a proteolytic activity of 58.31 U/mL and a collagenolytic activity of 28 U/mL. It presented a 5.755-fold purification with a recovery yield of 3.190%, enzymatic activity of 20.018 U/mL, and a specific activity of 25,904 U/mg. The lyophilized powder formulation showed greater resistance to degradation, maintaining approximately 68% residual activity, whereas the liquid formulation exhibited higher susceptibility to activity loss. Therefore, the drying method proved effective in improving enzyme stability, enabling longer use and supporting its potential future application in veterinary medicine.

COMMITTEE MEMBERS:
Presidente - TATIANA SOUZA PORTO
Externa ao Programa - ***.888.094-** - LILIANA ANDREA DOS SANTOS - UFRPE
Externo à Instituição - THIAGO PAJEÚ NASCIMENTO - UFPI