Banca de QUALIFICAÇÃO: ELIANA NUNES PEREIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ELIANA NUNES PEREIRA
DATE: 30/10/2025
TIME: 09:00
LOCAL: virtual
TITLE:

Development and characterization of a veterinary nanotechnological diluent based on essential oil

nanoemulsion for semen cryopreservation

KEY WORDS:

Animal Reproduction; Nanotechnology; Sperm Cell

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Inseminação Artificial Animal
SUMMARY:

Semen cryopreservation is a crucial biotechnological tool for the preservation of genetic resources,

reduction of costs associated with the transport and maintenance of breeding animals, and mitigation

of the spread of sexually transmitted diseases. However, the freezing and thawing processes can

compromise sperm fertility due to cryoinjuries, which negatively impact post-thaw pregnancy rates.

To minimize such damage, the use of appropriate extenders, cryoprotectants, and species-specific

freezing protocols is essential. Cryopreservation is also associated with an imbalance in reactive

oxygen species (ROS), leading to oxidative stress, structural damage, and decreased fertilization

capacity. In this context, natural compounds with antioxidant properties—such as essential oils

(EOs)—represent a promising alternative to enhance sperm protection. When incorporated into

nanoemulsions, these compounds can achieve controlled release and reduce cytotoxicity, thereby

improving their efficacy during the cryopreservation process. Nanotechnology, particularly the use

of nanoemulsion-based delivery systems, has shown significant potential in veterinary medicine and

animal reproduction. Nanoemulsions, characterized by droplet sizes of up to 200 nm, improve the

bioavailability and absorption of active compounds, in addition to offering physicochemical stability

and controlled substance release. This study aimed to develop and evaluate a nanoemulsion

containing an antioxidant-rich essential oil, to be incorporated into a semen freezing extender for

breeding animals. The objective was to reduce sperm membrane damage, thereby improving motility

and fertilization potential post-thaw. As an innovative approach utilizing essential oil in a

conventional extender (Tris-egg yolk) for the cryopreservation of animal spermatozoa, this study is

expected to enhance sperm preservation outcomes, increase the average number of viable

insemination doses, and contribute to genetic improvement of livestock herds. In the broader

context, this advancement may support increased productivity in animal husbandry, job creation,

and income generation.

COMMITTEE MEMBERS:
Presidente - MARIA MADALENA PESSOA GUERRA
Interno - GUSTAVO FERRER CARNEIRO
Interno - VALDEMIRO AMARO DA SILVA JUNIOR
Externa ao Programa - 2201997 - ELLEN CORDEIRO BENTO DA SILVA - UFRPE