Research on Indicators of Antimicrobial Resistance of Escherichia Coli in Ground Beef
Escherichia coli; Antimicrobial Resistance; Ground Beef; ESBL; Public Health
The preventive use of antimicrobials in livestock, especially as growth promoters, has raised public health concerns due to the risk of dissemination of resistant bacteria, such as Escherichia coli, to humans. This study aims to investigate indicators of antimicrobial resistance of E. coli in ground beef sold in supermarkets in Recife-PE. Forty samples collected aseptically will be analyzed and submitted to bacterial identification and antimicrobial susceptibility testing by disk diffusion on Muller Hinton agar. Colonies will be identified on EMB agar, with subsequent analysis of resistance profiles to antibiotics commonly used in human and veterinary medicine, including beta-lactams, quinolones and aminoglycosides. Samples positive for ESBL production will be subjected to DNA extraction and analysis by conventional PCR. It is expected to evaluate the frequency of resistance and contribute to the understanding of the presence of resistance genes in commercialized meat, proposing control and prevention measures for risks to public health.