Banca de QUALIFICAÇÃO: LUÍS EDUARDO RANGEL BATINGA DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LUÍS EDUARDO RANGEL BATINGA DE OLIVEIRA
DATE: 11/11/2024
TIME: 10:00
LOCAL: Departamento de Biologia
TITLE:

ANALYSIS OF  IFN-Y AND IL-10 CYTOKINES EXPRESSIONS IN PLACENTAL TISSUE OF COWS WITH AND WITHOUT HYPERKETONEMIA

KEY WORDS:

Hypertonemia, Infections, Expression, Placental Tissues, Cytokines.

PAGES: 57
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Patologia Clínica Animal
SUMMARY:

Hyperketonemia is a common condition in ruminant metabolism characterized by the increase of ketone bodies in tissues and fluids body. This syndrome has a significant impact on cattle farming, as it is linked to herd profitability and economic losses. Studies indicate a relationship between the development of metabolic disorders, such as hyperketonemia, and changes in cytokine levels. These changes can decrease inflammation, promoting metabolic homeostasis and, consequently, reduce the risk of chronic metabolic diseases. IFN-y is a cytokine with pro-inflammatory properties involved in the immune response, acting in inflammatory and autoimmune processes. In the placental tissues from humans, it has been observed to have a prominent role in protecting against infections from bacterial and viral pathogens. IL-10 is an anti-inflammatory cytokine that plays an important role in the regulation of the immune response and maintenance of homeostasis. In the placenta, it plays a fundamental role in the creation of an immunotolerant environment at the maternal-fetal interface. The present study aimed to evaluate whether the expression of IFN-y and IL-10 cytokines in the placenta of dairy cows was related to the development of hyperketonemia. A total of 31 mRNA samples from the placental tissue of cows were analyzed, 14 of them with hyperketonemia and 17 healthy dairy herds, all from dairy herds in the dairy basin of the municipality of Garanhuns, in the state of Pernambuco. The cDNA was synthesized followed of the expression level of the IFN-y and IL-10 genes using Real-time PCR software. Mean INF-y expression was lower in the hyperketonemia group compared to the control group (0.244 ± 2.07 vs 1.57 ± 1.36, respectively). On the other hand, in relation to IL-10, there was a higher expression in the group with hyperketonemia compared to the control group (0.0136 ± 2.83 vs -1.91 ± 2.25, respectively) showing statistically significant difference between them (p < 0.05). In addition, significant correlations were observed between the following variables: BHB with IFN-y; BHB with NEFAs; Albumin with IL-10; and Albumin with globulin. Thus, it is concluded that the production of pro-inflammatory and anti-inflammatory cytokines has relevant contributions to the well-being and health of ruminant animals that are in a transition period, being fundamental for physiological homeostasis of metabolism.

 

COMMITTEE MEMBERS:
Presidente - PAULO ROBERTO ELEUTERIO DE SOUZA
Interno - FABIO DE SOUZA MENDONCA
Interno - PABYTON GONCALVES CADENA
Externa ao Programa - 2887132 - ELAYNE CRISTINE SOARES DA SILVA - UFRPE