Banca de QUALIFICAÇÃO: MARCELO HONORATO DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCELO HONORATO DA SILVA
DATE: 07/10/2024
TIME: 09:00
LOCAL: Sala de seminario DMV
TITLE:

FREQUENCY AND MOLECULAR CHARACTERIZATION OF Ancylostoma caninum and Ancylostoma braziliense IN FECAL SAMPLES FROM DIFFERENT ORIGINS

KEY WORDS:

FLOTAC, Parasites, Zoonoses, Molecular Diagnosis, Canis lupus familiaris,

PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Parasitic diseases continue to pose a significant challenge to both animal and human health in the 21st century. In Brazil, the zoonotic genus Ancylostoma is particularly prevalent. The species Ancylostoma caninum and Ancylostoma braziliense are of veterinary medical interest in the country, as their larval stages are responsible for Cutaneous Larva Migrans in humans. Unfortunately, coproparasitological diagnosis of the genus Ancylostoma does not allow for differentiation between species in infected dogs due to the difficulty in visually distinguishing the eggs. The aim of this study was to diagnose natural infection by Ancylostoma caninum and Ancylostoma braziliense in dogs, primarily using the multivalent FLOTAC technique, to evaluate the frequency of gastrointestinal parasites in samples from both domestic and shelter animals, and subsequently to perform Polymerase Chain Reaction (PCR) analysis. In summary, 60 fecal samples were collected from dogs of different breeds and ages and assessed using the FLOTAC technique. Data were analyzed using descriptive statistics. Of the 60 samples processed by the FLOTAC coproparasitological technique, 38.3% (23/60) were from shelter animals, and 61.7% (37/60) were from domestic animals, both in the city of Recife. In this study, 46.7% (28/60) of the samples tested positive for zoonotic parasites, with Ancylostoma spp. being the most common at 40% (24/60), followed by Trichuris spp. at 8.3% (5/60), Toxocaraspp. at 6.7% (4/60), and Cystoisospora spp. at 1.7% (1/60). A relative frequency of coinfection (presence of two or more parasites in the same sample) was observed in 10% (6/60) of the analyzed samples. The frequency of parasitic infections in shelter animals was 78.3% (18/23), while in domestic animals, it was 27% (10/37), which is considered high. The genus Ancylostoma was identified in 40% (24/60) of the evaluated samples, with a frequency of 21.6% (8/37) in domestic dog samples and 69.6% (16/23) in shelter dog samples. Techniques based on DNA detection are necessary for species differentiation. To better understand parasitic agents, it is essential to have knowledge of their epidemiology, distribution, and species identification according to geographical regions

COMMITTEE MEMBERS:
Interna - GILCIA APARECIDA DE CARVALHO
Interno - LEUCIO CAMARA ALVES
Externa à Instituição - MARIA FERNANDA MELO MONTEIRO