The objectives of the study were to detect infection by Leishmania infantum in dogs  using different biological samples, and to carry out epidemiological surveillance of Visceral Leishmaniasis in an area of the City of Recife with a human case. Domestic, adult, male and female dogs, of different ages and breeds, treated at the parasitic diseases outpatient clinic of the Federal Rural University of Pernambuco (UFRPE), with a positive parasitological diagnosis and without prior treatment for CanL (Canine Leishmaniasis) underwent blood collection for serological tests, DPP (Dual path platform) and IFA (Indirect Immunofluorescence assay), and collection of Conjunctival swab (CS), Precorneal tear film (PCTF), Nasal swab (NS) and Throat swab (TS) for molecular diagnosis using PCR (Polymerase Chain Reaction). When compared the CS-PCR with that of FLPC, CS displayed better performance (p = 0.4489) than samples from PCTF, although without statistical difference. Analyzing the IFA results of animals where the IFA titer was equal to or greater than 1/160, the CS-PCR detected the largest number of positive samples, although without statistical difference (p = 0.4797). For the NS and TS results, the NS-PCR detected a greater number of positive samples, with a significant statistical difference (p = 0.0368). Therefore, the use of less invasive collections represents less cost and good sensitivity for diagnosis through PCR. This was the first study using PCR with a filter paper strip to collect tear film for the molecular diagnosis of CanL. In this study, a serological survey was also carried out on dogs from the Passarinho neighborhood, Recife-PE, after the detection of a human case of Visceral Leishmaniasis. Collections were made from 205 domestic, male and female dogs, of different ages and breeds, to perform DPP as a triage test, and EIE (enzyme immunoassay) for the confirmatory test. There was no confirmation of CanL in the studied neighborhood.