Banca de DEFESA: FRANCINE MARIA DE FRANCA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FRANCINE MARIA DE FRANCA SILVA
DATE: 26/02/2024
TIME: 08:30
LOCAL: https://meet.google.com/fts-bwxy-xta
TITLE:

Epidemiological and therapeutic study of feline sporotrichosis in the Metropolitan Region of Recife

KEY WORDS:

Sporothrix brasiliensis; Cat; Georeferencing; Treatment; Refractoriness; Resistance.

PAGES: 120
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The objective of this study was to evaluate the clinical, epidemiological, therapeutic characteristics, and in vitro susceptibility to Sporothrix brasiliensis in felines in the Metropolitan Region of Recife (RMR). The cats were attended at the Veterinary Hospital of the Federal Rural University of Pernambuco, and information was obtained through questionnaires. The animals were diagnosed by fungal culture, and those meeting the inclusion criteria were subjected to different treatment protocols. Minimum inhibitory concentration (MIC) was used to assess the in vitro susceptibility of the isolates to itraconazole (ITZ), amphotericin B (ANF), terbinafine (TBF), and potassium iodide (KI). Molecular biology using PCR identified the S. brasiliensis species in all 110 (100%) samples. The epidemiological profile highlighted a higher prevalence of semi-domesticated adult male cats with other feline contacts and no prior treatment for sporotrichosis. The cities of Recife and Camaragibe had the highest concentrations of cases. Ulcers, crusts, sneezing, nasal swelling, and dyspnea were the most prevalent outcomes. The association of clinical forms, disseminated cutaneous and mucocutaneous, was predominant, especially in the ears, nose, and pelvic limbs. Regarding therapeutic variables, animals in the ITZ group and those with predominance of skin lesions showed the highest cure rate, while animals treated with ITZ + KI had the highest frequency of death. ITZ, ANF, and TBF showed good in vitro susceptibility, while KI showed high MIC values. Two non-WT isolates for ITZ and six non-WT isolates for TBF were identified. It is concluded that this study of epidemiological behavior in the RMR can be used as a tool for the development of management and prophylaxis measures. The in vitro susceptibility pattern was compatible with the good therapeutic response of felines treated with ITZ. The MIC values of ITZ and ANF were influenced by the use of previous antifungals without increasing the frequency of non-WT isolates. Further studies involving isolates with high MIC values and clinical correlation are necessary to investigate other causes of refractoriness and to better understand the disease's behavior in vivo. 

COMMITTEE MEMBERS:
Presidente - JOSE WILTON PINHEIRO JUNIOR
Externa ao Programa - 2240345 - EDNA MICHELLY DE SA SANTOS - UFRPEExterna à Instituição - BRUNA RODRIGUES DE SOUSA - UFPE
Externo à Instituição - CARLOS ADRIANO DE SANTANA LEAL
Externo à Instituição - LEONILDO BENTO GALIZA DA SILVA - UFRA