Banca de QUALIFICAÇÃO: NATALY SAYONARA DA SILVA MELO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : NATALY SAYONARA DA SILVA MELO
DATE: 20/12/2023
TIME: 09:00
LOCAL: PPGBA UFRPE - meet
TITLE:

MOLECULAR CHARACTERIZATION OF Escherichia coli AND Staphylococcus aureus ISOLATES FROM ARTISAN COALHO CHEESE SOLD NEAR FAMILY HEALTH UNITS

KEY WORDS:

resistance genes, antimicrobials, enterotoxins, waterborne and foodborne diseases

PAGES: 100
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Inspeção de Produtos de Origem Animal
SUMMARY:

Food contamination is a public health and economic problem, due to the risks of contamination by microorganisms that can cause diseases in the population, known as Water and Foodborne Diseases, in addition to the deterioration of the food itself. Among the most reported microorganisms in cases of Foodborne outbreaks are Staphylococcus aureus and Escherichia coli, which are opportunistic microorganisms that can be spread between humans, animals and the environment, and even in food. These bacterial agents also play an important role in the spread of resistance to antimicrobials, mainly through mobile genetic elements, which has been considered an important public health problem worldwide. Artisanal coalho cheese is one of the foods that can be contaminated by these microorganisms, as it is produced with raw bovine milk and undergoes considerable manipulation in its production and commercialization. This product is widely consumed in the Northeast region of Brazil, mainly in the state of Pernambuco. Due to a greater possibility of contamination by S. aureus and E. coli, the objective of this work was to molecularly characterize strains of S. aureus and E. coli resistant to antimicrobials isolated from samples of artisanal coalho cheese sold in establishments located nearby to the eight health districts of the city of Recife - PE. Of the 129 samples collected, S. aureus was identified in 49 samples, and E. coli was detected in samples using advanced molecular techniques. The confirmed isolates were then subjected to phenotypic resistance tests, such as the Kirby-Bauer disk diffusion test and the minimum inhibitory concentration (MIC) test. The spa-typing method was used to type S. aureus isolates, and the most detected spa-types were t11521 (23.91%), t701 (17.39%) and t359 (6.52%). After molecular confirmation, the isolates had their genome sequenced. In S. aureus isolates, the enterotoxin genes sei, seq, sem, seo, seu, sen, sek, and seg were detected, representing 2.17% of the isolates, respectively, and seh (8.69%). The virulence genes hlgA (100%), hlgB (100%), hlgC (100%), lukD (97.82%), lukE (97.82%), lukF-PV and lukS-PV (4.34%) both, and tst (2.17%), were also detected. In E. coli isolates, the virulence genes identified were csgA (100%), fdeC (66.66%), fimH (66.66%), gad (66.66%), hlyE (100%), iss (100%), lpfA (33.33%), terC (100%), sitA (66.66%), yehA (66.66%), yehB (66.66%), YehC (100%) and yehD (100%). Regarding resistance genes in all E. coli isolates, the blaEC gene was detected. In relation to S. aureus, resistance genes were detected in 98.22% of the isolates, with the blaZ (53.57%) and tet(38) (80.35%) genes being the most frequent, the mecA gene was detected in 4.44% and the msrA gene in 4.44% of isolates. The presence of these microorganisms in foods, such as artisanal coalho cheese, can have a major impact on public health, as they can be disseminated in different environments and transfer their virulence characteristics, such as enterotoxin genes, which can lead to death, and resistance that can trigger resistance to different antimicrobial agents, and consequently lead to failures in disease treatments.

COMMITTEE MEMBERS:
Presidente - TATIANA SOUZA PORTO
Interno - JOSE WILTON PINHEIRO JUNIOR
Externo à Instituição - JOSÉ GIVANILDO DA SILVA - UFBA