Banca de QUALIFICAÇÃO: MATHEUS VICTOR VIANA DE MELO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MATHEUS VICTOR VIANA DE MELO
DATE: 23/10/2023
TIME: 10:00
LOCAL: Online via Google Meet
TITLE:

Development of a feed with nanoemulsion for sex reversal of tilapia (Oreochromis niloticus)

KEY WORDS:

Tilapia, nanotechnology, sex reversal, hormone.

PAGES: 33
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Continuous investments are made to boost the development of the animal production market. Aquaculture stands out in this context, producing 88 million tons of aquatic animals in 2020. In Brazil, with fish exports in 2022, a value of 23.8 million dollars was generated, with tilapia accounting for 98% of that export, contributing to the country being the fourth-largest producer of this fish in the world. Tilapia offers production advantages, with males being preferred due to their size and better production control. However, to ensure male monosex, various technologies are developed, such as the addition of androgenic hormones to the feed, which, if not done carefully and controlled, can lead to pollution and environmental contamination. A safer alternative is the use of controlled-release systems, such as nanoemulsions. Thus, the aim of this study was to produce and characterize a nanoscale nanoemulsion containing encapsulated 17α-methyltestosterone, associated with tilapia Oreochromis niloticus feed to induce sex reversal. The nanoemulsion followed a previously developed protocol. Organoleptic and physicochemical parameters were analyzed. The animals went through different growth phases, divided into 4 groups: negative control, fed only with feed; positive control, fed with feed and a commercial veterinary product for masculinization; and groups with 30 mg and 60 mg of testosterone in nanoemulsion added to the feed. At the end, their gonads were analyzed to confirm their sex. The results indicate that all nanoemulsion samples were homogeneous, opaque, without color, odor, and lumps, except for sample 5, which was slightly thick. Moreover, it was possible to produce samples with nanovesicles with a good polydispersion index and a satisfactory zeta potential value. The groups subjected to sex reversal achieved over 99% success in sex reversal, larger morphometric measurements than the control, and reduced mortality in the nanoemulsion-treated groups. Thus, it can be concluded that it is possible to use a nanoemulsion system with encapsulated androgenic hormone for efficient sex reversal of tilapia with increased safety and the potential for reducing environmental impacts.

COMMITTEE MEMBERS:
Interno - PAULO ROBERTO ELEUTERIO DE SOUZA
Externa à Instituição - MARIA DO CARMO DE BARROS PIMENTEL - UFPE