Banca de QUALIFICAÇÃO: DANIELY OLIVEIRA DO NASCIMENTO ROCHA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : DANIELY OLIVEIRA DO NASCIMENTO ROCHA
DATE: 15/09/2023
TIME: 15:00
LOCAL: https://meet.google.com/eea-kcec-mzb
TITLE:

Filarioids in dogs from a costal area of the state of Pernambuco

KEY WORDS:

Dirofilaria immitis, Cercopithifilaria bainae, Acanthocheilonema reconditum, Beach area, Vectors, Zoonosis.

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Filarial are nematode parasites that affect different species of animals, including domestic dogs, wild canids, felines and humans. Among the species of medical and veterinary importance for dogs, we can highlight the genera Dirofilaria and Acanthocheilonema, found in the blood, and the microfilariae that inhabit the skin represented by the genera Cercopithifilaria and Onchocerca. These parasites are trans-mitted through the blood-feeding of arthropod vectors (e.g., mosquitoes, fleas, lice and ticks). This study aimed to determine the prevalence of blood and dermal filarial infection in dogs residing in a coastal area of the State of Pernambuco. From February to September 2021, blood samples (n = 245) were collected and stored in plastic tubes containing an anticoagulant (ethylenediamine tetracetic acid - EDTA) for the modified Knott test, followed by the qualitative ELISA test (SNAP® 4Dx® Plus, IDEXX Laboratory, Westbrook, Maine, USA) and thus detect antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato, Ehrlichia spp. and Dirofilaria immitis antigens. Fragments of skin samples (n = 71) collected with a disposable punch were included in this study. Subsequently, these skin fragments were examined microscopically and molecularly evaluated through PCR targeting the 12S rDNA gene. Microfilariae and D. immitis antigen were detected in 24 (9.8%; 95% CI = 6.7–14.2) animals. In the ELISA test, 9 (3.67%) microfilaremic animals had negative results. Co-infection among Dirofilaria immitis and other pathogens pre-dominated over isolated infections by D. immitis (χ2 = 4.381; p = 0.0363). No filarial was detected in the skin after microscopic and molecular analyses. Dogs positive for D. immitis were found in four different municipalities, including two (Cabo de Santo Agostinho and Ipojuca), where there is an intense flow of animals and humans attracted by touristic activities in these localities. Data from this study demonstrate that D. immitis is the main filarial nematode infecting dogs in northeastern Brazil's beach areas. Preventive measures such as repellent collars and microfilaricidal compounds should be used to prevent canine infection associated with correct environmental management in these areas to control vector populations.

COMMITTEE MEMBERS:
Presidente - RAFAEL ANTONIO DO NASCIMENTO RAMOS
Interna - GILCIA APARECIDA DE CARVALHO
Externa à Instituição - LUCIA OLIVEIRA DE MACEDO
Externa à Instituição - MARCIA PAULA OLIVEIRA FARIAS - UFPI