Banca de DEFESA: YASMIM BARBOSA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : YASMIM BARBOSA DOS SANTOS
DATE: 27/02/2023
TIME: 08:00
LOCAL: Recife - PE
TITLE:

STUDY OF THE IMMUNOMODULATORY EFFECT OF MELATONIN ON THE THYMUS AND SPLEEN IN THE OFFSPRING OF RATS EXPOSED TO CHRONIC ALCOHOL CONSUMPTION DURING PREGNANCY AND LACTATION

KEY WORDS:

apoptosis, immunity, rat, fetus, pineal, oxidative stress, alcohol

PAGES: 81
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Consumption of alcohol during pregnancy interferes with the development of the fetus and results in a number of adverse effects. This abusive use of alcohol can compromise the immune response and cause changes in organs such as the thymus, spleen, liver and kidneys, since one of the main reasons for the damage induced by alcohol in embryonic and fetal development is its pro-oxidant effect, which leads to increased oxidative stress. Due to its antioxidant, immunomodulatory and anti-inflammatory roles, melatonin can protect cells, DNA and mitochondria against damage caused by ROS. However, the role played by this hormone during pregnancy and lactation is not known. Thus, this work aims to evaluate the effects of exogenous melatonin on the thymus, spleen, liver and kidneys of offspring whose sows were subjected to chronic alcohol consumption during pregnancy and lactation. For this purpose, 30 female albino rats (Rattus norvegicus albinus) from the Animal Facility of the DMFA (Department of Animal Morphology and Physiology), UFRPE, were administered 3 g/Kg of alcohol intragastrically and treated with melatonin. (0.8 mg/Kg) were started on the first day of pregnancy until the end of lactation. They were divided to form the following experimental groups: Control group - Pups of rats that did not receive alcohol during pregnancy and lactation; Alcohol group - Pups of rats subjected to chronic alcohol consumption during pregnancy and lactation; Alcohol + Mel Group - Pups of rats subjected to chronic alcohol consumption and simultaneously treated with melatonin during pregnancy and lactation. The animals were euthanized at 30 days of life, for collection of the organs that were fixed and underwent routine histological processing and morphometric, histopathological, immunohistochemical and organosomatic index analyzes were performed. In the results of histopathology and morphometry, beneficial effects were observed in the alcohol+mel group, since the spleen did not present a reduction in the area of the white pulp or a large amount of hematopoietic cells, in the thymus, these same animals also maintained the size of the area thymic, unlike the alcohol group. In immunohistochemistry, the alcohol+mel and control groups showed weak IL-1β staining and a low apoptotic index in the thymus and spleen when compared to the alcohol group, in addition to not altering the organosomatic index for both organs. In the alcohol+mel group, congestion in the portal and centrilobular veins and steatosis was avoided in the liver, in addition to not showing changes in the size of the hepatic parenchyma. While in the kidneys, melatonin did not change the size of the glomeruli and maintained the subcapsular space, in addition to avoiding congestion in the cortical area. Furthermore, the alcohol+mel group also did not show a decrease in the weight and length of the offspring at birth, unlike the alcohol group. Thus, we conclude that melatonin can attenuate damage by free radicals in organs such as the thymus, spleen, liver and kidneys, modulating the apoptotic index, body and organ weight, histological changes and the level of pro-inflammatory cytokines in the offspring of mothers who consume alcohol during pregnancy and lactation.

COMMITTEE MEMBERS:
Presidente - VALERIA WANDERLEY TEIXEIRA
Interno - ALVARO AGUIAR COELHO TEIXEIRA
Externa à Instituição - FERNANDA DAS CHAGAS ÂNGELO MENDES TENÓRIO - UFPE