Banca de DEFESA: GABRIELA HÉMYLIN FERREIRA MOURA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : GABRIELA HÉMYLIN FERREIRA MOURA
DATE: 28/07/2022
TIME: 08:30
LOCAL: Remoto
TITLE:

Study of infection by Toxoplasma gondii and other protozoa of the Sarcocystidae family in birds and wild animals from the states of Pernambuco and Rio Grande do Norte

KEY WORDS:

Protozoa; Isospora spp; Sarcocystis spp.; Wild animals; Caatinga; Atlantic forest

PAGES: 94
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

The objective was to evaluate the occurrence of IgG anti-Toxoplasma gondii antibodies and the DNA of protozoa of the Sarcoscystidae family in birds and wild mammals from the Caatinga biome and from the Pernambuco Wild Animal Screening Center (CETRAS-Tangara) in the Atlantic Forest. In the first study, the investigation of antibodies against T. gondii and DNA of protozoa of the family Sarcocystidae was carried out in 55 free-living animals from the Caatinga biome using the MAT technique and nested PCR followed by sequencing, respectively in 55 samples of mammals from three wild species. In the second study, the DNA research of protozoa of the Sarcocystidae family was carried out in 96 captive birds from CETRAS-Tangara and free-living birds from the Caatinga biome, totaling 96 animals from 41 species. In the third study, DNA research of the Sarcocystidae family was carried out in 79 mammals of five species from the Caatinga biome and the CETRAS-Tangara. To identify the protozoa of the Sarcocystidae family in the three studies, nested PCR was used for the 18s rDNA gene followed by genetic sequencing. In the first study, antibodies were detected in 7/29 (24.13%) armadillos, 6/16 (37.5%) collared peccaries and absence of antibodies in agoutis 0/10 and Toxoplasma gondii DNA in 6/ 19 (31.57%) of the giant armadillos and no collared peccary were positive. In the second study, 25% (24/96) of the bird samples were positive for the protozoa: Isospora spp., Sarcocystis spp. and Toxoplasma gondii in eight bird species (Amazona aestiva, Coereba flaveola, Egretta thula, Paroaria dominicana, Sporophila nigricollis, Cariama cristata, Columbina talpacoti, Crypturellus parvirostris). In the third study, 25.31% (20/79) of samples from nine species of mammals (Conepatus semistriatus, Didelphis albiventris, Tamandua tetradactyla, Bradypus variegatus, Sapajus libidinosus, Thrichomys laurentius, Monodelphis domestica, Galea spix and Callithrix jaccus) were positive, for T. gondii. The occurrence of antibodies and DNA of the Sarcocystidae family in birds and wild mammals of the Caatinga and CETRAS-Tangara biomes demonstrates the participation of these species in the wild cycle of the identified protozoa and the need to intensify research in this area for the conservation of wild animals in the Caatinga and Atlantic Forest biomes.

BANKING MEMBERS:
Interno - 2118675 - RINALDO APARECIDO MOTA
Externa ao Programa - 1076653 - RENATA PIMENTEL BANDEIRA DE MELO
Externa à Instituição - DÉBORA COSTA VIEGAS DE LIMA - UFPI
Externa à Instituição - CECILIA IRENE PEREZ CALABUIG - UFERSA
Externa à Instituição - DEBORA ROCHELLY ALVES FERREIRA