Banca de QUALIFICAÇÃO: ANA CLAUDIA CARVALHO DE SOUSA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA CLAUDIA CARVALHO DE SOUSA
DATE: 13/05/2022
TIME: 09:00
LOCAL: Plataforma on-line
TITLE:

ACTION OF MELATONIN ON THE LIVER AND KIDNEYS IN TYLOXAPOL-INDUCED HYPERLIPIDEMIC RATS

KEY WORDS:

melatonin, cytokines, hyperlipidemia, liver, kidney

PAGES: 63
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Hyperlipidemia, also known as dyslipidemia, is characterized by an increase in triglyceride and LDL levels and a reduction in HDL levels, which may be a result of primary factors, of a genetic nature, or secondary, as a result of external causes such as a diet rich in fat, sedentary lifestyle, smoking, and obesity. The relationship with cardiovascular diseases makes hyperlipidemia a public health problem, which can lead to death or physical disability. The liver is the organ responsible for lipid and cholesterol homeostasis, being extremely important in biochemical regulation and lipid homeostasis. The peroxidation of these lipids leads to the formation of reactive oxygen species (ROS), which when in excess can lead to the activation of genes for the production of inflammatory mediators, such as tumor necrosis factor alpha (TNF-α) and interleukins (IL), that can lead to dysregulation of cellular homeostasis, especially in high metabolic organs such as liver and kidneys. Melatonin has a protective role against oxidative stress, being a potent free radical scavenger, thus protecting cells and tissues from damage. Thus, the present research aimed to evaluate the effects of melatonin on the liver and kidneys of hyperlipidemic rats induced by tyloxapol. Fifteen animals were used in 3 groups: Control Group (CG): animals without hyperlipidemia induction; Hyperlipidemic group (TG); Hyperlipidemic group treated with Melatonin (GTM). Hyperlipidemia was induced by intraperitoneal injection, for fifteen interleaved days, of Triton WR 1339, also known as tyloxapol (Sigma Aldrich), at a dose of 400mg/Kg. Melatonin was administered in daily injections for 15 days, intraperitoneally at a dose of 20 mg/kg. Biochemical analyzes at 7 days of treatment revealed that there were no significant differences between the GT and GTM groups in relation to LDL and VLDL, although treatment with melatonin reduced serum levels of triglycerides, cholesterol, alkaline phosphatase, TGP, and TGO. At 15 days of treatment, it was found that treatment with melatonin led to a reduction in the levels of all biochemical parameters. The histopathological analysis showed that in the group treated only with tyloxapol, steatosis and leukocyte infiltrate were observed, while the group treated with tyloxapol and melatonin showed no changes in the liver parenchyma. non-lobular, present in the GT group. Melatonin treatment also considerably reduced lobular and hepatocyte inflammation, which was observed in the hyperlipidemic group. Immunohistochemical analysis revealed a reduction in the expression of inflammatory cytokines and an increase in anti-inflammatory in the group treated with melatonin.

BANKING MEMBERS:
Interno - 384942 - ALVARO AGUIAR COELHO TEIXEIRA
Externa à Instituição - ISMAELA MARIA FERREIRA DE MELO
Presidente - 2308550 - VALERIA WANDERLEY TEIXEIRA