Banca de DEFESA: GABRIELA REIS XAVIER

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GABRIELA REIS XAVIER
DATE: 26/02/2026
TIME: 09:00
LOCAL: Auditório de Reprodução Animal – DMV/UFRPE
TITLE:

Microbiological isolation and antimicrobial resistance patterns in mares with infectious endometritis in Pernambuco

KEY WORDS:

Infectious endometritis, Antimicrobial resistance, Cervical conformation

PAGES: 46
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

Infectious endometritis is one of the main causes of subfertility in mares and is associated with the persistence of microorganisms within the uterine lumen, an inadequate inflammatory response, and anatomical factors that impair the natural uterine defense mechanisms. Among these factors, cervical conformation plays a fundamental role in proper uterine drainage and in preventing bacterial ascension and persistence. Therefore, the aim of this study was to perform the microbiological isolation and identification of bacteria from the uterus of mares diagnosed with infectious endometritis in the state of Pernambuco, Brazil, to evaluate the antimicrobial resistance profile, the occurrence of multidrug resistance, the ability to form biofilm, and to relate these findings to cervical conformation and intrauterine bacterial persistence. Uterine samples were collected from mares with positive uterine cytology and subjected to bacterial isolation and identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Antimicrobial susceptibility was determined using the disk diffusion method, while biofilm production was assessed by a phenotypic assay. Cervical conformation was clinically classified according to its relationship with uterine drainage capacity. A total of 47 bacterial isolates were obtained, with a predominance of Gram-negative bacteria. Escherichia coli was the most frequently isolated species, accounting for 53.2% of the isolates, followed by Citrobacter koseri (12.8%) and Proteus mirabilis (8.5%). Regarding biofilm formation, 51.1% of the isolates showed some degree of biofilm production, classified as weak (34.0%), moderate (10.6%), or strong (6.4%). Multidrug-resistant isolates, defined as those resistant to three or more antimicrobials, represented 25.5% of the total evaluated. A higher occurrence of bacterial persistence was observed in mares with unfavorable cervical conformation, particularly those classified in classes associated with reduced uterine drainage efficiency. These results demonstrate that infectious endometritis in mares is a multifactorial condition in which the interaction between bacterial agents, antimicrobial resistance, biofilm formation, and cervical conformation contributes to the maintenance of infection. These findings reinforce the importance of an integrated diagnostic approach combining clinical reproductive evaluation and laboratory analyses to establish more effective therapeutic strategies in equine reproduction.

COMMITTEE MEMBERS:
Presidente - GUSTAVO FERRER CARNEIRO
Externo à Instituição - ANDRE DE SOUZA SANTOS
Externo à Instituição - GILSON SANTOS BUONORA