Banca de DEFESA: JERLANE TARCILIA GOMES TELLES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JERLANE TARCILIA GOMES TELLES
DATE: 20/02/2026
TIME: 09:00
LOCAL: Auditório do prédio de sanidade e tecnologia de caprinos e ovinos do Departamento de Medicina Veteri
TITLE:

Isolation, molecular characterization, and development of a kit applied to the immunodiagnosis of Bovine Enzootic Leukosis

KEY WORDS:

Cattle, epidemiology, viral genetics, veterinary medicine, virology.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Bovine Enzootic Leukosis (BLE) is a notifiable infectious disease with a significant economic impact due to production losses and associated trade restrictions. In Brazil, the lack of national diagnostic kits and the dependence on imported tests limit large-scale testing, highlighting the need for accessible methods. This research aims to perform the first isolation and characterization of Bovine Leukemia Virus (VLB) in the State of Pernambuco and to develop and evaluate an Agar Gel Immunodiffusion (AGID) kit for the serological diagnosis of BLE. A serum biobank was established, and the antigen was produced in persistently infected Fetal Lamb Kidney (FLK) cells, with confirmation of the presence of VLB and absence of Bovine Viral Diarrhea Virus (BVDV) by two-phase Polymerase Chain Reaction (Nested-PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR), respectively. In total, 360 samples and were previously classified by Enzyme-Linked Immunosorbent Assay (ELISA-IDEXX®). The IDGA showed clear precipitation lines, sensitivity of 87.1%, specificity of 98%, observed concordance of 0.90, and a Kappa index of 0.86. In parallel, the first isolation of VLB in Pernambuco was carried out, confirmed by Nested-PCR, sequencing of the env gene (gp51), and phylogenetic analysis, which revealed the isolate's clustering in genotype 1. These findings broaden the knowledge about viral diversity in the Northeast and reinforce the importance of continuous genomic surveillance in Brazil, furthermore, the developed immunodiagnostic kit demonstrated satisfactory analytical and diagnostic performance

COMMITTEE MEMBERS:
Presidente - JOSE WILTON PINHEIRO JUNIOR
Externa ao Programa - 2478437 - LUCIANA DE OLIVEIRA FRANCO - UFRPEExterna à Instituição - POLLYANNE RAYSA FERNANDES DE OLIVEIRA