Banca de QUALIFICAÇÃO: MARIA DE NAZARE SANTOS FERREIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIA DE NAZARE SANTOS FERREIRA
DATE: 11/12/2025
TIME: 08:30
LOCAL: Videoconferência (https://meet.google.com/ujd-qysy-hwt)
TITLE:

Epidemiological-molecular study of Parapoxvirus orf isolates from goats and sheep and study of the perception of rural producers and veterinarians regarding contagious ecthyma in the state of Pernambuco, Brazil

KEY WORDS:

Contagious Ecthyma Virus; Small Ruminants; PCR; Perception Study; One Health.

PAGES: 56
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

The objective of this research was to conduct an epidemiological and molecular study of Parapoxvirus orf in goat and sheep herds in the state of Pernambuco, Brazil. In addition, to conduct an analysis of the perception of veterinarians and rural producers in Pernambuco regarding contagious ecthyma. Crust samples were collected from 15 goats and 44 sheep distributed across properties in the Agreste, Sertão, and Metropolitan regions of the state of Pernambuco. Of the 59 scab samples, 100% were positive for the B2L gene of Parapoxvirus orf using the Polymerase Chain Reaction (PCR) technique. Phylogenetic analysis revealed six main clades, demonstrating genetic diversification and alignment with strains previously described in Brazil and worldwide. Regarding viral isolation, 69.50% (41/59) of the samples were subjected to cell culture, and of these, 85.36% (35/41) showed cytopathic effects. At the end of the isolation, the supernatants of the 41 samples were analyzed by PCR, and 100% were positive for the B2L gene. It is concluded that Parapoxvirus orf persists with a pattern of endemicity in goat and sheep species on farms in the state of Pernambuco, presenting phylogenetically multiple active strains and patterns of infectivity in vitro, highlighting the urgency of clinical, epidemiological, and molecular surveillance strategies aligned with the concept of One Health.

COMMITTEE MEMBERS:
Presidente - JOSE WILTON PINHEIRO JUNIOR
Externa ao Programa - 1647041 - RITA DE CASSIA CARVALHO MAIA - UFRPEExterno à Instituição - RODOLFO DE MORAES PEIXOTO