Banca de QUALIFICAÇÃO: JHONATAS MATEUS DA SILVA ALVES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JHONATAS MATEUS DA SILVA ALVES
DATE: 26/09/2025
TIME: 14:00
LOCAL: Google meet
TITLE:

ACTION OF MELATONIN ON THE LIVER OF RATS SUBJECTED TO THE INSECTICIDE BIFENTRIN

KEY WORDS:

Hepatotoxicity; Pyrethroids; Antioxidants; Immunohistochemistry; Biochemistry; Pesticides

PAGES: 60
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Pyrethroids are the leading pesticides, widely used in agriculture and in household insecticides and sanitizing products. Among these compounds, bifenthrin has gained increasing popularity and market share, being associated with potential toxic effects on non-target organisms, especially the liver, the main organ of xenobiotic biotransformation. Given the risks associated with prolonged exposure, antioxidant strategies have proven relevant in mitigating this damage. Melatonin, with its recognized hepatoprotective action, emerges as a promising alternative. In this context, this study aims to evaluate the hepatic toxic effects of bifenthrin and investigate its potential protective effect in an experimental model with rats. Thirty 60-day-old female Wistar rats were divided into the following experimental groups: Control; BIF3mg/kg; BIF6mg/kg; BIF3mg/kg+Mel10mg/kg; and BIF6mg/kg+Mel10mg/kg. Bifenthrin was administered by gavage and melatonin intraperitoneally, both for 25 consecutive days. At the end, histopathological, histochemical, morphometric, and immunohistochemical analyses were performed for markers of liver injury and the organosomatic index was assessed. Statistical analysis of the data revealed that exposure to bifenthrin promoted liver histopathological alterations, including hepatocellular ballooning, centrilobular vein congestion, microgoticular steatosis, lobular inflammation, leukocyte infiltration, and disorganization of hepatocyte cords around the centrilobular veins, with greater severity at the 6 mg/kg dose. Morphometric analysis indicated an increase in lobular parenchyma and a reduction in non-lobular parenchyma in the exposed groups, an effect attenuated by melatonin. Histochemical analysis revealed an increase in collagen deposition in the livers of these animals, while there was a reduction in the liver organosomatic index in the BIF3 mg/kg and BIF6 mg/kg groups. Biochemical analyses showed significant increases in LDL, cholesterol, alkaline phosphatase, ALT, and AST, as well as a reduction in HDL in these groups. Melatonin treatment in the BIF3 mg+MEL 10 mg/kg and BIF6 mg+MEL 10 mg/kg groups normalized these parameters compared to the control group. Immunohistochemical analyses are still ongoing, and the results will be included in the final version of the study.

 

COMMITTEE MEMBERS:
Presidente - VALERIA WANDERLEY TEIXEIRA
Interno - ANISIO FRANCISCO SOARES
Externo à Instituição - JAIURTE GOMES MARTINS DA SILVA