Banca de QUALIFICAÇÃO: LUCIANA KELLY DE SOUSA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCIANA KELLY DE SOUSA SILVA
DATE: 27/09/2024
TIME: 10:00
LOCAL: Remoto (ON LINE)
TITLE:

REPRODUCTIVE BIOLOGY OF Tropidurus semitaeniatus Spix, 1825 (Squamata, Tropiduridae) ANALYZED IN TWO BIOMAS

KEY WORDS:

Reproduction. Lizards. Caatinga. Atlantic Forest.

PAGES: 43
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUMMARY:

Regarding animal reproduction, it is believed that various reproductive strategies have evolved to avoid predation, minimize competition, or simply adapt to environmental conditions. Thus, knowledge of the reproductive biology of lizards becomes relevant, as it constitutes a crucial factor for understanding the evolutionary process, along with species adaptations in response to environmental pressures. In this context, when considering the Atlantic Forest biome, characterized as a semideciduous seasonal forest, and the Caatinga biome, with a semi-arid climate, it is important to investigate the reproductive biology of lizards in these regions, understanding the influence of climatic factors on the regulation of reproductive cells, and comparing the reproductive cycle of the species Tropidurus semitaeniatus. In this way, the present project aims to characterize and compare histologically and morphometrically the gonads, livers, and fat bodies of the species Tropidurus semitaeniatus collected in Caatinga and Atlantic Forest environments, verifying the effects of environmental factors on their reproductive process. The collection was carried out in the Olho d'Água das Onças Ecological Reserve, in Picuí-PB (Caatinga environment), and in the Tapacurá Ecological Station, in São Lourenço da Mata-PE (Atlantic Forest). Monthly expeditions were conducted over four consecutive days throughout a year, with three daily outings. A maximum of 8 animals were collected in each area, euthanized with 5% lidocaine, measured with a digital caliper, weighed, identified, and fixed in 10% formaldehyde. For the hepatosomatic (HSI), gonadosomatic (GSI), and liposomatic (LSI) indices, the weight and measurements of the gonads, livers, and fat bodies were collected. After fixation, the material was dehydrated in an ascending selective alcohol series, cleared, embedded in paraffin, sectioned at 5.0 μm, and stained with Hematoxylin and Eosin. For the calculation of the volume density (Vv) of spermatids and spermatozoa, the formula of Mandarim-de-Lacerda (1991) and Weibel (1979) was used. To quantify profile density, fields were counted in a Test Area (TA) of 0.044 mm² for each animal. The final result (mm²) was obtained using the average of each profile, applying the formula: QA = Ʃprofiles/TA. The quantification of oocyte types was carried out through the population density method in a Test Area of 88 mm². The values of reproductive lineage cells were analyzed over the months using the Kruskal-Wallis test and, when necessary, complemented by Dunn's Test. For dimorphism, general measurements of the animals were taken and submitted to Principal Component Analysis (PCA). The results obtained for the condition factor (k1) and body growth indices in relation to environmental variables were subjected to Spearman's demonstration test and multiple linear regression. The correlation of k1 with the indices was performed using Spearman's correlation coefficient test. The dependence between cell density and occurrence was verified by a two-way ANOVA test. Rainfall data were obtained from the database of the Weather Forecast and Climate Studies Center and by using a thermohygrometer. Sexual dimorphism was found for the species in both areas, with males showing higher morphometric variable measurements than females. The body condition factor  k1 and the GSI, HSI, and LSI indices varied throughout the months of collection in both areas. However, only the HSI of Caatinga females and the LSI and GSI of Atlantic Forest males and females were significant. In the analysis of the correlation between the condition factor and the evaluated indices, only the GSI and HSI of Atlantic Forest males and females, as well as the HSI of Caatinga males and females, showed statistical significance. On the other hand, the interference of Caatinga's rainfall and temperature on the development of k1 and our indices were not statistically significant.

COMMITTEE MEMBERS:
Presidente - GERALDO JORGE BARBOSA DE MOURA
Externo à Instituição - WASHINGTON LUIZ DA SILVA VIEIRA - UFPB
Externo à Instituição - DANIEL OLIVEIRA MESQUITA - UFPB