Banca de DEFESA: RENATO AMORIM DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RENATO AMORIM DA SILVA
DATE: 27/02/2024
TIME: 08:00
LOCAL: https://meet.google.com/efs-jiqx-jhh
TITLE:

Identification and isolation of Toxoplasma gondii genotypes from chickens (Gallus domesticus) obtained from local markets in Pernambuco state, Brazil.

KEY WORDS:

Apicomplexa, bioassay, genotyping, public health, zoonosis

PAGES: 54
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Brazil has several economically profitable activities, among which the agricultural axis stands out. The raising of chickens (Gallus domesticus) to obtain animal products is carried out at varying levels of technology, with the birds being particularly exposed to infectious agents when raised in a free-living environment in direct contact with soil. Toxoplasma gondii is an obligatory intracellular parasite with worldwide distribution and capable of parasitizing several homeothermic species, and which is of great importance due to its zoonotic potential. Considering the low technnification of family chicken-farming environments, which often allows contact between the definitive host of Toxoplasma gondii and domestic chickens, it is important to evaluate the circulation of this parasite amongst this animal population and, also, its viability. The aim of this study was to isolate and identify T. gondii from tissues of domestic chickens raised extensively and intended for human consumption. Blood and tissue samples were collected from 25 chickens destined for slaughter. Initially, the Indirect Immunofluorescence Assay (IFAT) technique was used as screening to detect antibodies anti-T. gondii. The positive animals at the cutoff point of 1:16 had their tissues (brain, heart and liver) subjected to the Bioassay in a murine model, using Swiss-Webster mice. The inoculated mice were evaluated for a 45 days period, and on the thirtieth day post-inoculation, a blood sample was collected from each inoculated mouse to detect antibodies anti-T. gondii by IFAT. Animals were euthanized on day 45 p.i. and those positive at the cutoff point of 1:16 had their brains evaluated for T. gondii cysts and subsequently inoculated into an in vitro culture of MA-104 (ATCC® CRL-2378.1) cells. Aliquots of mouse tissue and cell culture were subjected to Polymerase Chain Reaction (PCR) for confirmation through detection of the ITS-1 gene. A frequency of 68.00% (17/25) of anti-T. gondii in the evaluated chickens, which resulted in a seroconversion of 64.70% (22/34) in the inoculated mice. Seven T. gondii isolates were obtained, confirmed by molecular assays. From the results obtained, the presence and viability of T. gondii cysts in chickens intended for human consumption is reported. This occurrence reflects the status of environmental contamination, providing data regarding the circulation of a zoonotic agent of severe importance for public health.

 

COMMITTEE MEMBERS:
Presidente - JOSE WILTON PINHEIRO JUNIOR
Externa à Instituição - THAIS FERREIRA FEITOSA
Externo à Instituição - WAGNNER JOSE NASCIMENTO PORTO