Banca de DEFESA: VALÉRIA MILENA DANTAS DE CASTRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : VALÉRIA MILENA DANTAS DE CASTRO
DATE: 16/02/2024
TIME: 14:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

EFFECTS OF HYPERBARIC OXYGEN THERAPY IN THE DUODENUM OF RATS WITH
STREPTOZOTOCIN-INDUCED DIABETES

KEY WORDS:

Hyperbaric Oxygen; Diabetes Mellitus; Duodenal Mucosa; Morphometry; Inflammation; Oxidative Stress.

PAGES: 86
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Diabetes Mellitus (DM) is a metabolic disorder that results in persistent hyperglycemia
resulting from a lack of insulin or failures in its action. This disorder is generally
accompanied by changes in cellular redox state signaling pathways that result in
inflammatory responses and subsequently in morphological damage to tissues and
organs, complications associated with diabetes. Given the damage that DM causes to
patients, many studies have been testing alternative therapies that can reduce these
complications, such as tissue hyperoxygenation. Hyperbaric Oxygen Therapy (HBOT)
involves inhaling pure oxygen at high pressure. From this, the objective of this work is
to evaluate the effects of (HBOT) on the pre-clinical, morphological, inflammatory and
oxidative parameters of the duodenum of diabetic rats. In this work, 32 Wistar rats
aged 60 days, weighing around 220-270g, were used. The animals remained housed in
polypropylene boxes where they received food and water ad libitum. The sample was
distributed into four experimental groups: control group composed of healthy animals
(C), control group treated with hyperbaric oxygen therapy (C+HBOT), diabetic group
without treatment (D) and diabetic group treated with oxygen therapy (D+HBOT). It
was used inside the chamber at 2.5 absolute atmospheres (ATA) at a rate of 2
ATA/min and maintained at that pressure for 60 min. The chamber was ventilated
with 100% O2 at a flow rate of 20 L/min in order to minimize CO2 accumulation. After
euthanasia, the entire duodenum was removed, where 5 centimeters were allocated
for histomorphometric, immunohistochemical and stereological analyzes and 2
centimeters for marking reactive oxygen species (ROS) with a DHE probe. It was found
that HBOT improved polyphagia in the D+HBOT group versus D, reversing changes in
the reference volume of the duodenal lumen and absolute volume of the submucosa.
It also improved the expression of Caspase-3, VEGF, SOD-1 AND GPX in the D+HBOT
group versus D. From the results we concluded that hyperoxygenation in this study
was not able to reduce blood glucose, but had positive effects at the tissue level,
reducing morphological changes in volume as well as improving the expression of
proteins involved in apoptosis, inflammation and oxidative stress.

COMMITTEE MEMBERS:
Externo à Instituição - GILBERTO SANTOS CERQUEIRA - UFC
Externa à Instituição - KARINA CARLA DE PAULA MEDEIROS - UFRN
Interno - FABIO DE SOUZA MENDONCA
Presidente - JOAQUIM EVENCIO NETO
Externa à Instituição - NAISANDRA BEZERRA DA SILVA FARIAS