Banca de QUALIFICAÇÃO: CAMILLA DE ANDRADE TENORIO CAVALCANTI

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : CAMILLA DE ANDRADE TENORIO CAVALCANTI
DATE: 11/12/2023
TIME: 09:00
LOCAL: Prédio da Biologia
TITLE:

MURINOMETRIC, BEHAVIORAL AND BIOCHEMICAL ANALYZES OF WISTAR RATS IN A DNBS-INDUCED ULCERATIVE RETOCOLITIS MODEL: AN INTEGRATED ASSESSMENT OF RELATED PARAMETERS.

KEY WORDS:

Psychiatric disorders; colitis; oxidative stress; DNBS.

PAGES: 79
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

Mental disorders are complex, multifactorial conditions, and current research is focused on understanding the role of inflammation and the immune system in their pathophysiology. In this context, increased intestinal permeability plays a central role in the systemic inflammatory response, influencing the microbiota-gut-brain axis. This indicates a link between gastrointestinal health and emotional regulation, with significant implications for understanding and treating mental disorders. Therefore, the main objective of this research was to evaluate behavioral, physiological, and molecular changes in an animal model of intestinal inflammation induced by 4,6-dinitrobenzenesulfonic acid (DNBS). To achieve this goal, 20 rats (males, aged 60 days) were subjected to induction of ulcerative colitis by rectal administration of DNBS (40 mg diluted in 50% ethanol). Subsequently, the animals underwent a battery of behavioral tests: open field, light-dark transition, forced swimming, and rotarod. Finally, biological material collection was performed - colon, hippocampus, striatum, and cortex - for biochemical tests, namely oxidative stress and western blot. DNBS-induced colitis was characterized by changes in colon weight and length, as well as the presence of inflammation, ulcers, and necrosis. Another relevant aspect is the significant weight loss compared to the control. Regarding behavioral tests, an increase in grooming in the Open Field Test, associated with an increase in immobility time in the Forced Swimming Test, was noteworthy. Oxidative stress was characterized by an increase in MDA and a decline in GSH and NP-SH levels. Thus, the tests conducted in this study revealed that the induction of experimental colitis through DNBS resulted in manifestations of depressive and anxious behavior in animals, as well as alterations in the colon.

 

 

COMMITTEE MEMBERS:
Presidente - ROMILDO DE ALBUQUERQUE NOGUEIRA
Interno - VALDEMIRO AMARO DA SILVA JUNIOR
Externo à Instituição - BRUNO MENDES TENORIO - UFPE